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AB211461

Anti-Syntaxin 7 antibody [CL0257]

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(1 Publication)

Mouse Monoclonal Syntaxin 7 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STX7 aa 1-200.

View Alternative Names

Syntaxin-7, STX7

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody [CL0257] (AB211461)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody [CL0257] (AB211461)

Immunohistochemical analysis of human lymph node tissue labeling Syntaxin 7 with ab211461 at 1/500 dilution.

Shows strong membrane positivity in non-germinal center cells.

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 7 antibody [CL0257] (AB211461)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 7 antibody [CL0257] (AB211461)

Immunocytochemistry/Immunofluorescence analysis of U-251 cells labelling Syntaxin 7 with ab211461 at 2μg/ml.

Shows specific staining in vesicles in green. Microtubule and nuclear probes are visualized in red and blue, respectively.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody [CL0257] (AB211461)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody [CL0257] (AB211461)

Immunohistochemical analysis of human melanoma tissue labeling Syntaxin 7 with ab211461 at 1/500 dilution.

Shows strong cytoplasmic and membrane positivity in tumour cells.

Western blot - Anti-Syntaxin 7 antibody [CL0257] (AB211461)
  • WB

Supplier Data

Western blot - Anti-Syntaxin 7 antibody [CL0257] (AB211461)

All lanes:

Western blot - Anti-Syntaxin 7 antibody [CL0257] (ab211461) at 1/500 dilution

All lanes:

Human tonsil tissue lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0257

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human STX7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O15400

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 7 also known as STX7 is a member of the SNARE protein family important for vesicle fusion processes. With a molecular mass of approximately 29 kDa Syntaxin 7 plays a significant role in the intracellular trafficking system by mediating membrane fusion events. It is expressed predominantly in many tissues including the brain and spleen where it contributes to diverse cellular functions. As a member of the target membrane SNARE proteins Syntaxin 7 partners with other SNARE proteins to facilitate endolysosomal transport.
Biological function summary

Syntaxin 7 mediates membrane fusion events essential for endosome-lysosome fusion. It forms a complex with other SNARE proteins like Vti1b Syntaxin 8 and VAMP8. This complex orchestrates the accurate trafficking and degradation of macromolecules within cells. Syntaxin 7's presence proves essential in ensuring molecules are delivered to lysosomes for degradation and it participates in recycling cellular components. This role helps maintain cellular homeostasis by regulating the turnover of cellular substances.

Pathways

Syntaxin 7 plays vital roles in processes related to vesicle transport and autophagy. It contributes significantly to the endocytic pathway an important pathway responsible for importing molecules into cells and directing them to lysosomes for breakdown. Syntaxin 7 is also involved in the autophagic-lysosomal pathway working in conjunction with proteins like Syntaxin 8 to facilitate autophagosome and lysosome fusion. Its coordination in these pathways highlights the importance of Syntaxin 7’s role in maintaining cellular health and balance.

Syntaxin 7 has been linked to conditions related to defective lysosomal storage and neurodegenerative diseases. In Alzheimer’s disease for instance impaired endosomal and lysosomal functions are typical and dysfunction of proteins like Syntaxin 7 can exacerbate these issues. Similarly abnormal expression of Syntaxin 7 could contribute to neuronal loss observed in Alzheimer's disease. Its interaction with other SNARE proteins in this context suggests that targeting Syntaxin 7 and its complex could offer therapeutic potential for addressing related cellular dysfunctions in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
See full target information STX7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33215216

2020

Osteoglycin knockdown promotes vascular smooth muscle cell proliferation and migration in aortic dissection via the VEGF/VEGFR2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zanxin Wang,Xianmian Zhuang,Bailang Chen,Minxin Wei
View all publications
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