Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Syntaxin with ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Negative control : no staining on human kidney. The section was incubated with ab188583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Syntaxin with ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on human cerebrum. The section was incubated with ab188583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IP

Supplier Data

Immunoprecipitation - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Human fetal brain lysates using ab188583. Detection of Syntaxin utilised ab188583 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>)

Predicted band size: 33 kDa

false

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling Syntaxin with ab188583 at 1/100 dilution (10.85 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). ab11267 Anti-MAP2 mouse monoclonal antibody was used for counterstaining at 1/500 dilution (4ug/ml) with counterstain secondary antiobdy ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution (2µg/mL) (Red). The Nuclear counterstain was DAPI (Blue). -ve control 1 : ab188583 used at 1/100 dilution with counterstain secondary antibody only ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution. -ve control 2 : ab11267 used at 1/500 dilution with target secondary antibody only ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 dilution. Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Syntaxin with ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on mouse cerebrum. The section was incubated with ab188583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Syntaxin with ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on rat cerebrum. The section was incubated with ab188583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling Syntaxin with ab188583 at 1/100 dilution (10.85 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). ab11267 Anti-MAP2 mouse monoclonal antibody was used for counterstaining at 1/500 dilution (4ug/ml) with counterstain secondary antiobdy ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution (2µg/mL) (Red). The Nuclear counterstain was DAPI (Blue). -ve control 1 : ab188583 used at 1/100 dilution with counterstain secondary antibody only ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution. -ve control 2 : ab11267 used at 1/500 dilution with target secondary antibody only ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 dilution. Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• WB

Supplier Data

Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>) at 1/50000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>) at 1/50000 dilution

All lanes:

Human glioma tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 33 kDa

false

• WB

Lab

Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Negative control : human heart, human kidney and human spleen. In Western blot, anti-GAPDH antibody (ab181602) staining at 1/20, 0000 dilution.

All lanes:

Western blot - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Negative control : rat heart, rat kidney and rat spleen. In Western blot, anti-GAPDH antibody (ab181602) staining at 1/20, 0000 dilution.

All lanes:

Western blot - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975)

This data was developed using ab188583, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Negative control : mouse heart, mouse kidney and mouse spleen. In Western blot, anti-GAPDH antibody (ab181602) staining at 1/20, 0000 dilution.

All lanes:

Western blot - Anti-Syntaxin antibody [EPR15139(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-antibody-epr15139b-ab188583'>ab188583</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 1s