Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)

Rabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (AB250975), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested	Expected
Mouse	Expected	Tested	Tested	Tested
Rat	Expected	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information STX1B

Function

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm (By similarity).

Alternative names

STX1B1, STX1B2, STX1B, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2

Recommended products

Rabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR15139(B)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab250975 is the carrier-free version of Anti-Syntaxin antibody [EPR15139(B)] ab188583.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary: Syntaxin also known as HPC-1 or STX1A functions as an important component in the vesicle docking and fusion process particularly in neuronal exocytosis. This protein weighs approximately 33 kDa and has a significant presence in the brain especially in the synapses. Syntaxin belongs to the SNARE protein family and plays a pivotal role in facilitating the fusion of synaptic vesicles with the presynaptic membrane allowing neurotransmitter release. Its expression is not limited to nervous tissue but occurs in various other tissues reflecting its versatile roles in cellular activities.
Biological function summary: Syntaxin interacts with other SNARE proteins such as SNAP-25 and VAMP to form the SNARE complex essential for synaptic vesicle fusion and neurotransmitter release. This complex mediates the membrane fusion process vital for effective neuronal communication. Syntaxin exhibits interaction with several regulatory proteins like Munc18 which stabilizes its closed conformation critical in maintaining a reservoir of readily releasable vesicles. Its involvement in the SNARE complex marks its importance in cellular exocytosis and endocytosis.
Pathways: Syntaxin participates prominently in the neurotransmission and secretion pathways. It interacts within the neurotransmission pathway by associating with SNAP-25 and VAMP facilitating rapid neurotransmitter release in synaptic transmission. Additionally in the secretion pathway Syntaxin involvement extends to insulin release interacting with proteins like Munc18 and enhancing vesicular transport processes. Its action in these pathways demonstrates its fundamental role in cellular signaling and material transport.
Associated diseases and disorders: Syntaxin's dysfunction has links to neurological conditions such as Alzheimer's disease and botulism. In Alzheimer's disease abnormal Syntaxin interaction may impair synaptic function and neurotransmitter release leading to cognitive deficits. Botulism caused by botulinum toxin disrupts Syntaxin-mediated vesicle docking leading to muscular paralysis. Related proteins like SNAP-25 also play a role in these conditions emphasizing the complex network within the SNARE pathway that Syntaxin operates within highlighting its significance in maintaining cellular homeostasis and function.

Product promise

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12 product images

Immunoprecipitation - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human fetal brain lysates using Anti-Syntaxin antibody [EPR15139(B)] ab188583. Detection of Syntaxin utilised Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583)
Predicted band size: 33 kDa
Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583) at 1/50000 dilution
All lanes: Human fetal brain tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583) at 1/50000 dilution
All lanes: Human glioma tissue lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Positive staining on human cerebrum. The section was incubated with Anti-Syntaxin antibody [EPR15139(B)] ab188583 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

Negative control: human heart, human kidney and human spleen.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/20, 0000 dilution.
All lanes: Western blot - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Human kidney tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1s
Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

Negative control: mouse heart, mouse kidney and mouse spleen.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/20, 0000 dilution.
All lanes: Western blot - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1s
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/100 dilution (10.85 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used for counterstaining at 1/500 dilution (4ug/ml) with counterstain secondary antiobdy Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution (2µg/mL) (Red). The Nuclear counterstain was DAPI (Blue). -ve control 1: Anti-Syntaxin antibody [EPR15139(B)] ab188583 used at 1/100 dilution with counterstain secondary antibody only Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution. -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 used at 1/500 dilution with target secondary antibody only Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 dilution.

Confocal image showing positive staining in rat primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Positive staining on mouse cerebrum. The section was incubated with Anti-Syntaxin antibody [EPR15139(B)] ab188583 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

Negative control: rat heart, rat kidney and rat spleen.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/20, 0000 dilution.
All lanes: Western blot - Anti-Syntaxin antibody [EPR15139(B)] (Anti-Syntaxin antibody [EPR15139(B)] ab188583) at 1/1000 dilution
Lane 1: Rat cerebellum tissue lysate at 20 µg
Lane 2: Rat heart tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Negative control: no staining on human kidney. The section was incubated with Anti-Syntaxin antibody [EPR15139(B)] ab188583 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/100 dilution (10.85 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used for counterstaining at 1/500 dilution (4ug/ml) with counterstain secondary antiobdy Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution (2µg/mL) (Red). The Nuclear counterstain was DAPI (Blue). -ve control 1: Anti-Syntaxin antibody [EPR15139(B)] ab188583 used at 1/100 dilution with counterstain secondary antibody only Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used at 1/1000 dilution. -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 used at 1/500 dilution with target secondary antibody only Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1/1000 dilution.

Confocal image showing positive staining in mouse primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free (ab250975)
This data was developed using Anti-Syntaxin antibody [EPR15139(B)] ab188583, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Syntaxin with Anti-Syntaxin antibody [EPR15139(B)] ab188583 at 1/10000 dilution (0.109 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Positive staining on rat cerebrum. The section was incubated with Anti-Syntaxin antibody [EPR15139(B)] ab188583 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.