Anti-Syntenin antibody (ab19903) is a rabbit polyclonal antibody that is used to detect Syntenin in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with Syntenin knockout cell line validation
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- Cell communication and signaling : CCS 21:2762023Assessment of technical and clinical utility of a bead-based flow cytometry platform for multiparametric phenotyping of CNS-derived extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Brahmer,Carsten Geiß,Andriani Lygeraki,Elmo Neuberger,Theophilos Tzaridis,Tinh Thi Nguyen,Felix Luessi,Anne Régnier-Vigouroux,Gunther Hartmann,Perikles Simon,Kristina Endres,Stefan Bittner,Katrin S Reiners,Eva-Maria Krämer-AlbersPubMed 37803478
- Journal of translational medicine 21:6522023FTO-targeted siRNA delivery by MSC-derived exosomes synergistically alleviates dopaminergic neuronal death in Parkinson's disease via m6A-dependent regulation of ATM mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Geng,Xinyi Long,Yuting Zhang,Yupeng Wang,Guoxing You,Wenjie Guo,Gaoming Zhuang,Yuanyuan Zhang,Xiao Cheng,Zhengqiang Yuan,Jie ZanPubMed 37737187
- International journal of molecular sciences 24:2023Identification of a Non-Invasive Urinary Exosomal Biomarker for Diabetic Nephropathy Using Data-Independent Acquisition Proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaonan Ding,Dong Zhang,Qinqin Ren,Yilan Hu,Jifeng Wang,Jing Hao,Haoran Wang,Xiaolin Zhao,Xiaochen Wang,Chenwen Song,Junxia Du,Fuquan Yang,Hanyu ZhuPubMed 37686366
- Human cell 36:1389-14022023Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yu,Xiaonan Wu,Wenyi Zhang,Fuhang Chu,Qi Zhang,Meihua Gao,Yingjie Xu,Yingtao WuPubMed 37269415
- Endocrine journal 70:635-6452023Chronic hyperadiponectinemia induced by transgenic overexpression increases plasma exosomes without significantly improving glucose and lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeitaro Kawada,Shunbun Kita,Shiro Fukuda,Keita Fukuoka,Tomonori Okita,Emi Kawada-Horitani,Masahito Iioka,Taka-Aki Sakaue,Yusuke Kawachi,Kohei Fujii,Yu Kimura,Shuichi Otabe,Yuya Fujishima,Hitoshi Nishizawa,Norikazu Maeda,Iichiro ShimomuraPubMed 37062722
- ACS nano 17:7562-75752023Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Hu,Shu Zhang,Yue Yang,Juan Li,Hongxin Kang,Wenfu Tang,Christopher J Lyon,Meihua WanPubMed 37022097
- Molecular therapy. Nucleic acids 31:541-5522023Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimón Álvarez,Pamina Contreras-Kallens,Sebastian Aguayo,Orlando Ramírez,Catalina Vallejos,Jorge Ruiz,Eva Carrasco-Gallardo,Stefanie Troncoso-Vera,Bernardo Morales,Christina M A P SchuhPubMed 36895953
- International journal of molecular sciences 24:2023Dapagliflozin Treatment Augments Bioactive Phosphatidylethanolamine Concentrations in Kidney Cortex Membrane Fractions of Hypertensive Diabetic db/db Mice and Alters the Density of Lipid Rafts in Mouse Proximal Tubule Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed F Gholam,Lauren P Liu,Louis A Searcy,Nancy D Denslow,Abdel A AlliPubMed 36674924
- International journal of molecular sciences 23:2022Lipid Profiles of Urinary Extracellular Vesicles Released during the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliana Pena Lopez,Mohammad-Zaman Nouri,Areej Ebrahim,Kevin M Chacko,Whitney C Schramm,Mohammed F Gholam,Tezcan Ozrazgat-Baslanti,Nancy D Denslow,Abdel A AlliPubMed 36499728
- JCI insight 7:2022Cell-free DNA topology depends on its subcellular and cellular origins in cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan Z Malkin,Steven De Michino,Meghan Lambie,Rita Gill,Zhen Zhao,Ariana Rostami,Andrea Arruda,Mark D Minden,Scott V BratmanPubMed 36125881
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Monkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Monkey | Dilution info - | Notes - |
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Target data
Function
Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:26291527). Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed:25893292). In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed:22660413). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed:11498591). May also play a role in vesicular trafficking (PubMed:11179419). Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed:10230395).
Alternative names
MDA9, SYCL, SDCBP, Syntenin-1, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, Syndecan-binding protein 1, MDA-9, TACIP18
Recommended products
Anti-Syntenin antibody (ab19903) is a rabbit polyclonal antibody that is used to detect Syntenin in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with Syntenin knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Syntenin antibody (ab19903) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Syntenin?
Anti-Syntenin (ab19903) specifically detects a band for Syntenin (UniProt: O08992) at a molecular weight of 32kDa.
Trusted by the scientific community
Anti-Syntenin (ab19903) was first used in a scientific publication in 2005 and has been cited over 40 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-Syntenin antibody (ab19903) has been confirmed by Western blot testing in SDCBP Knockout HAP1 cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntenin also known as syndecan binding protein (SDCBP) is a multifunctional adaptor protein with a molecular weight of approximately 32 kDa. It typically finds expression in various tissues with high levels noted in the brain placenta and kidney. Mechanically syntenin facilitates cellular signal transduction by interacting with the cytoplasmic domains of syndecans therefore playing a vital role in modulating those signals. Notably syntenin associates with the actin cytoskeleton impacting cell shape and motility.
- Biological function summary
Syntenin regulates important cellular processes such as cell adhesion migration and intracellular trafficking. It often associates with protein complexes like those formed with PDZ domain-containing proteins enhancing its ability to link and organize diverse cellular pathways. By influencing these cellular processes syntenin has an impact on tissue development and repair mechanisms. This protein's interaction with the cytoskeleton and membrane-bound molecules allows it to act as an essential mediator in cellular communication.
- Pathways
Syntenin is integral to the Wnt and TGF-beta signaling pathways which regulate cell growth and differentiation. Syntenin interacts with proteins such as Β-catenin and Smad3 within these pathways linking extracellular signaling cues to specific intracellular responses. These interactions highlight syntenin's role in modulating gene expression and maintaining cellular homeostasis especially in processes like embryogenesis and tissue regeneration.
- Associated diseases and disorders
Syntenin holds significance in cancer progression and neurodegenerative diseases. Its overexpression or altered function correlates with increased metastasis in various cancers associated with proteins like syndecan-1 and matrix metalloproteinases. In neurodegeneration dysregulated syntenin expression influences signaling pathways potentially contributing to disorders like Alzheimer's disease. Understanding syntenin's role in these conditions might lead to more targeted therapeutic strategies and improved disease management.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Syntenin antibody (ab19903)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Syntenin knockout HAP1 whole cell lysate (20 μg)
Lane 3: A549 whole cell lysate (20 μg)
Lane 4: HeLa whole cell lysate (20 μg)
Lanes 1-4: Merged signal (red and green). Green - ab19903 observed at 32 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
ab19903 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab19903 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Syntenin antibody (ab19903)
Predicted band size: 32 kDa
Western blot - Anti-Syntenin antibody (ab19903)
ab19903 detects a band of the expected size (32kDa) in HEK-293 cell lysate. This band is partially quenched by the addition of ab20431. No band is observed in human heart lysate.
All lanes: Western blot - Anti-Syntenin antibody (ab19903) at 1 µg/mL
Lane 1: HEK-293 cell lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: HEK-293 cell lysate at 20 µg with Mouse Syntenin peptide (ab20431)
Lane 4: Human heart tissue lysate at 20 µg with Mouse Syntenin peptide (ab20431)
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Western blot - Anti-Syntenin antibody (ab19903)
Syntenin Western blot staining using rabbit Anti-Syntenin antibody
ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate.
All lanes: Western blot - Anti-Syntenin antibody (ab19903) at 1 µg/mL
Lane 1: Mouse heart lysate at 20 µg
Lane 2: Rat heart lysate at 20 µg
Lane 3: Mouse heart lysate at 20 µg with Mouse Syntenin peptide (ab20431)
Lane 4: Rat heart lysate at 20 µg with Mouse Syntenin peptide (ab20431)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa, 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody (ab19903)
ICC/IF image of ab19903 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19903, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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