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Rabbit Polyclonal Syntenin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 46 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Monkey
Predicted
Predicted
Xenopus laevis
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Rat

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Xenopus laevis, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Xenopus laevis, Monkey

Dilution info

-

Notes

-

Target data

Function

Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:26291527). Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed:25893292). In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed:22660413). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed:11498591). May also play a role in vesicular trafficking (PubMed:11179419). Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed:10230395).

Alternative names

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Rabbit Polyclonal Syntenin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 46 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological summary

Syntenin regulates important cellular processes such as cell adhesion migration and intracellular trafficking. It often associates with protein complexes like those formed with PDZ domain-containing proteins enhancing its ability to link and organize diverse cellular pathways. By influencing these cellular processes syntenin has an impact on tissue development and repair mechanisms. This protein's interaction with the cytoskeleton and membrane-bound molecules allows it to act as an essential mediator in cellular communication.

Mechanical summary

Syntenin also known as syndecan binding protein (SDCBP) is a multifunctional adaptor protein with a molecular weight of approximately 32 kDa. It typically finds expression in various tissues with high levels noted in the brain placenta and kidney. Mechanically syntenin facilitates cellular signal transduction by interacting with the cytoplasmic domains of syndecans therefore playing a vital role in modulating those signals. Notably syntenin associates with the actin cytoskeleton impacting cell shape and motility.

Pathway

Syntenin is integral to the Wnt and TGF-beta signaling pathways which regulate cell growth and differentiation. Syntenin interacts with proteins such as β-catenin and Smad3 within these pathways linking extracellular signaling cues to specific intracellular responses. These interactions highlight syntenin's role in modulating gene expression and maintaining cellular homeostasis especially in processes like embryogenesis and tissue regeneration.

Disease

Syntenin holds significance in cancer progression and neurodegenerative diseases. Its overexpression or altered function correlates with increased metastasis in various cancers associated with proteins like syndecan-1 and matrix metalloproteinases. In neurodegeneration dysregulated syntenin expression influences signaling pathways potentially contributing to disorders like Alzheimer's disease. Understanding syntenin's role in these conditions might lead to more targeted therapeutic strategies and improved disease management.

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4 product images

  • Western blot - Anti-Syntenin antibody (ab19903), expandable thumbnail

    Western blot - Anti-Syntenin antibody (ab19903)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Syntenin knockout HAP1 whole cell lysate (20 μg)
    Lane 3: A549 whole cell lysate (20 μg)
    Lane 4: HeLa whole cell lysate (20 μg)

    Lanes 1-4: Merged signal (red and green). Green - ab19903 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab19903 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab19903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Syntenin antibody (AB19903)

    Predicted band size: 32 kDa

  • Western blot - Anti-Syntenin antibody (ab19903), expandable thumbnail

    Western blot - Anti-Syntenin antibody (ab19903)

    ab19903 detects a band of the expected size (32kDa) in HEK-293 cell lysate. This band is partially quenched by the addition of ab20431. No band is observed in human heart lysate.

    All lanes: Western blot - Anti-Syntenin antibody (AB19903) at 1 µg/mL

    Lanes 1 and 3: HEK-293 cell lysate at 20 µg

    Lanes 2 and 4: Human heart tissue lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-Syntenin antibody (ab19903), expandable thumbnail

    Western blot - Anti-Syntenin antibody (ab19903)

    ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate.

    All lanes: Western blot - Anti-Syntenin antibody (AB19903) at 1 µg/mL

    Lanes 1 and 3: Mouse heart lysate at 20 µg

    Lanes 2 and 4: Rat heart lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721) at 1/50000 dilution

    Predicted band size: 32 kDa

    Observed band size: 32 kDa, 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody (ab19903), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody (ab19903)

    ICC/IF image of ab19903 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19903, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

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Product protocols

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