Name: Anti-Syntenin antibody [EPR8102]
SKU: ab133267
Rating: 3 (1 reviews)

Anti-Syntenin antibody [EPR8102] ab133267 is a rabbit monoclonal antibody that is used in Syntenin western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR8102 is the most widely used clone for Syntenin on the market
- Specificity confirmed with SDCBP knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Images

Immunoprecipitation - Anti-Syntenin antibody [EPR8102] (AB133267), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	IP	WB	ICC/IF
Human	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/200	Notes -

Associated Products

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1 product for Alternative Product

Target data

See full target information SDCBP

Function

Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:26291527). Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed:25893292). In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed:22660413). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed:11498591). May also play a role in vesicular trafficking (PubMed:11179419). Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed:10230395).

Alternative names

MDA9, SYCL, SDCBP, Syntenin-1, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, Syndecan-binding protein 1, MDA-9, TACIP18

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR8102
Purification technique: Affinity purification Protein A
Dissociation constant: 1.24 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Syntenin also known as syndecan binding protein (SDCBP) is a multifunctional adaptor protein with a molecular weight of approximately 32 kDa. It typically finds expression in various tissues with high levels noted in the brain placenta and kidney. Mechanically syntenin facilitates cellular signal transduction by interacting with the cytoplasmic domains of syndecans therefore playing a vital role in modulating those signals. Notably syntenin associates with the actin cytoskeleton impacting cell shape and motility.

Biological function summary

Syntenin regulates important cellular processes such as cell adhesion migration and intracellular trafficking. It often associates with protein complexes like those formed with PDZ domain-containing proteins enhancing its ability to link and organize diverse cellular pathways. By influencing these cellular processes syntenin has an impact on tissue development and repair mechanisms. This protein's interaction with the cytoskeleton and membrane-bound molecules allows it to act as an essential mediator in cellular communication.

Pathways

Syntenin is integral to the Wnt and TGF-beta signaling pathways which regulate cell growth and differentiation. Syntenin interacts with proteins such as β-catenin and Smad3 within these pathways linking extracellular signaling cues to specific intracellular responses. These interactions highlight syntenin's role in modulating gene expression and maintaining cellular homeostasis especially in processes like embryogenesis and tissue regeneration.

Associated diseases and disorders

Syntenin holds significance in cancer progression and neurodegenerative diseases. Its overexpression or altered function correlates with increased metastasis in various cancers associated with proteins like syndecan-1 and matrix metalloproteinases. In neurodegeneration dysregulated syntenin expression influences signaling pathways potentially contributing to disorders like Alzheimer's disease. Understanding syntenin's role in these conditions might lead to more targeted therapeutic strategies and improved disease management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunoprecipitation - Anti-Syntenin antibody [EPR8102] (ab133267)
ab133267 (purified) at 1/40 immunoprecipitating Syntenin in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10μg)
Lane 2 (+): ab133267 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab133267 in HeLa whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Syntenin antibody [EPR8102] (ab133267)
Predicted band size: 32 kDa
Observed band size: 32 kDa
Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Syntenin knockout HAP1 whole cell lysate (20 μg)
Lane 3: A549 whole cell lysate (20 μg)
Lane 4: HeLa whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133267 observed at 32 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
ab133267 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab133267 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] (ab133267)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue labeling Syntenin with purified ab133267 at 1/50 dilution. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin
Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Syntenin antibody [EPR8102] (ab133267) at 1/2000 dilution
All lanes: A549 (Human lung carcinoma cell line) whole cell lysate at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Flow Cytometry (Intracellular) - Anti-Syntenin antibody [EPR8102] (ab133267)
Intracellular Flow Cytometry analysis ofHeLa (Human epithelial cell line from cervix adenocarcinoma)labeling Syntenin with purifiedab133267 at 1/50 (red).Cells were fixed with 4% paraformaldehyde. A goat anti rabbit IgG (Alexa Fluor^® 488) 1/2000 was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] (ab133267)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebral cortex tissue labeling Syntenin with purified ab133267 at 1/50 dilution. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Syntenin antibody [EPR8102] (ab133267) at 1/2000 dilution
Lane 1: Human placenta lysate at 15 µg
Lane 2: Human fetal brain lysate at 15 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 32 kDa, 46 kDa, 86 kDa
Observed band size: 32 kDa
Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Syntenin antibody [EPR8102] (ab133267) at 1/10000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 110 kDa, 119 kDa, 14 kDa, 15 kDa, 25 kDa, 32 kDa, 43 kDa
Observed band size: 11 kDa, 15 kDa, 25 kDa, 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody [EPR8102] (ab133267)
ab133267 staining Syntenin in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1: PBS only.
Flow Cytometry (Intracellular) - Anti-Syntenin antibody [EPR8102] (ab133267)
Overlay histogram showing SHSY-5Y cells stained with unpurified ab133267 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133267, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-Syntenin antibody [EPR8102] (ab133267)
All lanes: Western blot - Anti-Syntenin antibody [EPR8102] (ab133267) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: Human fetal heart lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] (ab133267)
Immunohistochemical analysis of Syntenin in paraffin embedded Human brain tissue, using unpurified ab133267 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Syntenin antibody [EPR8102] (ab133267)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] (ab133267)
Immunohistochemical analysis of formalin fixed paraffin embedded human liver carcinoma labelling Syntenin with ab133267 at a concentration of 0.01µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab133267 Anti-Syntenin antibody [EPR8102] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).