Rabbit Recombinant Monoclonal SYT1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--flow-cytometry-intracellular-img401223.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628)")
![Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--flow-cytometry-intracellular-img401222.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628)")
![Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-frozen-sections-img401248.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628)")
![Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-frozen-sections-img401247.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628)")
![Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunocytochemistry-immunofluorescence-img401204.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (AB302628)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Expected | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Tested | Tested | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Alternative names
SVP65, SYT, SYT1, Synaptotagmin-1, Synaptotagmin I, p65, SytI
Recommended products
Rabbit Recombinant Monoclonal SYT1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24488-129
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--flow-cytometry-intracellular-img401223.jpg "Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cell cells labelling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--flow-cytometry-intracellular-img401222.jpg "Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Flow Cytometry (Intracellular) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cell cells labelling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-frozen-sections-img401248.jpg "Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat liver (PMID: 24510058) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-frozen-sections-img401247.jpg "Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunohistochemistry (Frozen sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.![Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunocytochemistry-immunofluorescence-img401204.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/500 (0.964 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural/glia cell.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunocytochemistry-immunofluorescence-img401203.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/500 (0.964 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary neural/glia cell.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.![Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--western-blot-img401133.jpg "Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with mouse SYT2, SYT3, SYT4, SYT5, SYT6, SYT7, and SYT9.
These rec proteins were all made in-house and extracted from E.coil expression systems.
Exposure time: 15 secondsAll lanes: Western blot - Anti-SYT1 antibody [EPR24488-129] (Anti-SYT1 antibody [EPR24488-129] ab302627) at 1/1000 dilution
Lane 1: His-tagged mouse recombinant protein SYT1 (aa137-421) (35 kDa)
Lane 2: His-tagged mouse recombinant protein SYT2 (aa137-420) (34.8 kDa)
Lane 3: His-tagged mouse recombinant protein SYT3 (aa 76-587) (56.9 kDa)
Lane 4: His-tagged mouse recombinant protein SYT4 (aa 38-425) (45.6 kDa)
Lane 5: His-tagged mouse recombinant protein SYT5 (aa 46-386) (40.4 kDa)
Lane 6: His-tagged mouse recombinant protein SYT6 (aa 81-511) (51kDa)
Lane 7: His-tagged mouse recombinant protein SYT7 (aa 38-403) (43.6 kDa)
Lane 8: His-tagged mouse recombinant protein SYT9 (aa 74-491) (50.2 kDa)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 34.8 kDa, 56.9 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401172.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/20000 (0.024 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on rat testis. The section was incubated with Anti-SYT1 antibody [EPR24488-129] ab302627 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401171.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/20000 (0.024 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on nerve plexus of rat colon. The section was incubated with Anti-SYT1 antibody [EPR24488-129] ab302627 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401170.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Synaptotagmin-1 with Anti-SYT1 antibody [EPR24488-129] ab302627 at 1/20000 (0.024 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on rat cerebrum (PMID: 17190793). The section was incubated with Anti-SYT1 antibody [EPR24488-129] ab302627 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--western-blot-img401131.jpg "Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates of the right part (lane 2-5) were freshly made and used for Western blotting immediately to minimize protein degradation.
The band beneath the target band at approximately 37 kDa is likely to be degraded target fragments.
Negative controls: liver, testis, spleen (PMID:15561725, PMID:14715137)
Exposure time: 15 secondsAll lanes: Western blot - Anti-SYT1 antibody [EPR24488-129] (Anti-SYT1 antibody [EPR24488-129] ab302627) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate 20 µg
Lane 2: Mouse brain fresh tissue lysate 20 µg
Lane 3: Mouse liver fresh tissue lysate 20 µg
Lane 4: Mouse testis fresh tissue lysate 20 µg
Lane 5: Mouse spleen fresh tissue lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 60 kDa, 37 kDa
Exposure time: 15s
![Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--western-blot-img401132.jpg "Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)
This data was developed using Anti-SYT1 antibody [EPR24488-129] ab302627, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band at approximately 37 kDa are likely to be degraded target fragments.
Exposure time: Lane 1: 1 second
Lane 2-3: 7.75 secondsAll lanes: Western blot - Anti-SYT1 antibody [EPR24488-129] (Anti-SYT1 antibody [EPR24488-129] ab302627) at 1/1000 dilution
Lane 1: Rat brain tissue lysate 20 µg
Lane 2: Mouse spinal cord tissue lysate 20 µg
Lane 3: Human cerebellum tissue lysate 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 60 kDa, 37 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunocytochemistry-immunofluorescence-img401203.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401170.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
![Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--western-blot-img401131.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
![Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628), expandable thumbnail](https://content.abcam.com/products/images/syt1-antibody-epr24488-129-bsa-and-azide-free-ab302628--western-blot-img401132.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SYT1 antibody [EPR24488-129] (BSA and Azide free) (ab302628)")
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