JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181123

Anti-SYT2 antibody [EPR13204]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal SYT2 antibody. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

Synaptotagmin-2, Synaptotagmin II, SytII, SYT2

3 Images
Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)
  • WB

Lab

Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)

All lanes:

Western blot - Anti-SYT2 antibody [EPR13204] (ab181123) at 1/5000 dilution

All lanes:

Human cerebellum lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 67 kDa

false

Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)
  • WB

Unknown

Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)

This antibody has no cross-reacivity with mouse SYT1 , SYT3, SYT4, SYT5, SYT6, SYT7, and SYT9. These rec proteins were both made in house and extracted from E.coil expression systems.

All lanes:

Western blot - Anti-SYT2 antibody [EPR13204] (ab181123) at 1/1000 dilution

Lane 1:

His-tagged mouse recombinant protein SYT1 (aa137-421) at 0.03 µg

Lane 2:

His-tagged mouse recombinant protein SYT2 (aa137-420) at 0.03 µg

Lane 3:

His-tagged mouse recombinant protein SYT3 (aa 76-587) at 0.03 µg

Lane 4:

His-tagged mouse recombinant protein SYT4 (aa 38-425) at 0.03 µg

Lane 5:

His-tagged mouse recombinant protein SYT5 (aa 46-386) at 0.03 µg

Lane 6:

His-tagged mouse recombinant protein SYT6 (aa 81-511) at 0.03 µg

Lane 7:

His-tagged mouse recombinant protein SYT7 (aa 38-403) at 0.03 µg

Lane 8:

His-tagged mouse recombinant protein SYT9 (aa 74-491) at 0.03 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

false

Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)
  • WB

Lab

Western blot - Anti-SYT2 antibody [EPR13204] (AB181123)

Blocking/Diluting Buffer and concentration : 5% NFDM /TBST .

All lanes:

Western blot - Anti-SYT2 antibody [EPR13204] (ab181123) at 0.628 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg

Predicted band size: 47 kDa

Observed band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13204

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SYT2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 26:1365-1378 PubMed37429912

2023

Heart failure-induced cognitive dysfunction is mediated by intracellular Ca leak through ryanodine receptor type 2.

Applications

Unspecified application

Species

Unspecified reactive species

Haikel Dridi,Yang Liu,Steven Reiken,Xiaoping Liu,Elentina K Argyrousi,Qi Yuan,Marco C Miotto,Leah Sittenfeld,Andrei Meddar,Rajesh Kumar Soni,Ottavio Arancio,Alain Lacampagne,Andrew R Marks

American journal of physiology. Renal physiology 318:F457-F467 PubMed31760768

2019

Nitro-oleic acid inhibits the high glucose-induced epithelial-mesenchymal transition in peritoneal mesothelial cells and attenuates peritoneal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Su,Haiping Wang,ZiYan Feng,Jing Sun
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB154035

Anti-SYT2 antibody [ZNP-1]

Immunocytochemistry/ Immunofluorescence - Anti-SYT2 antibody [ZNP-1] (AB154035)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB302627

Anti-SYT1 antibody [EPR24488-129]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT1 antibody [EPR24488-129] (AB302627)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com