AB170901

Anti-SYVN1/HRD1 antibody [EP7459]

Name: Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) | Abcam
Brand: Abcam Limited
SKU: AB170901
Price: 250 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(25 Publications)

Rabbit Recombinant Monoclonal SYVN1/HRD1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 25 publications.

View Alternative Names

HRD1, KIAA1810, SYVN1, E3 ubiquitin-protein ligase synoviolin, RING-type E3 ubiquitin transferase synoviolin, Synovial apoptosis inhibitor 1

4 Images

Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1 using ab170901 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution

All lanes:

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

Predicted band size: 67 kDa

true

Supplier Data

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

All lanes:

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 67 kDa

true

578 PE

PE Anti-SYVN1/HRD1 antibody [EP7459]
Carrier free

Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7459

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed : 12459480, PubMed : 12646171, PubMed : 12975321, PubMed : 14593114, PubMed : 16289116, PubMed : 16847254, PubMed : 17059562, PubMed : 17141218, PubMed : 17170702, PubMed : 22607976, PubMed : 26471130, PubMed : 28827405). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 12459480, PubMed : 12646171, PubMed : 12975321, PubMed : 14593114, PubMed : 16289116, PubMed : 16847254, PubMed : 17059562, PubMed : 17141218, PubMed : 17170702, PubMed : 22607976, PubMed : 26471130, PubMed : 28842558). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed : 17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed : 17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (By similarity). During the early stage of B cell development, required for degradation of the pre-B cell receptor (pre-BCR) complex, hence supporting further differentiation into mature B cells (By similarity).

See full target information SYVN1

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1032-1046 PubMed40307574

2025

ELK1 inhibition alleviates amyloid pathology and memory decline by promoting the SYVN1-mediated ubiquitination and degradation of PS1 in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lilin Yi,Junjie Li,Yan He,Jiaojiao Wang,Maoju Wang,Song Guo,Man Luo,Bin Wu,Mingliang Xu,Qiuyun Tian,Yepeng Fan,Mulan Chen,Boqing Xu,Lei Xia,Weihong Song,Guiqiong He,Yehong Du,Zhifang Dong

Neurochemical research 49:117-128 PubMed37632637

2023

Luteolin Protects Against 6-Hydoroxydopamine-Induced Cell Death via an Upregulation of HRD1 and SEL1L.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Nishiguchi,Tomohiro Omura,Ayaka Sato,Yumi Kitahiro,Kazuhiro Yamamoto,Junichi Kunimasa,Ikuko Yano

iScience 26:107556 PubMed37664624

2023

LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Jing Zhang,Xiao-Lang Tan,Lei Guo

Cellular oncology (Dordrecht) 46:1285-1299 PubMed37099251

2023

SYVN1 ubiquitinates FoxO1 to induce β-catenin nuclear translocation, PD-L1-mediated metastasis, and immune evasion of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xie,Lei Shi,Hu Quan,Hua Xiao,Jie Chen,Jia Liu,Jean de Dieu Habimana,Rongqi Huang,Jia Luo,Pan Chen,Zhiyuan Li

Translational oncology 32:101649 PubMed36947996

2023

Downregulation of miR-221-3p promotes the ferroptosis in gastric cancer cells via upregulation of ATF3 to mediate the transcription inhibition of GPX4 and HRD1.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Jiang Shao,Hai-Lang Zhou,Xu-Zhu Gao,Chun-Fang Xu

Cell cycle (Georgetown, Tex.) 22:1116-1126 PubMed36809917

2023

HRD1 functions as a tumor suppressor in ovarian cancer by facilitating ubiquitination-dependent SLC7A11 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Wang,Shanfeng Wang,Wenjuan Zhang

Antioxidants (Basel, Switzerland) 12: PubMed36670935

2022

Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Wanying Liu,Ke Li,Menglin Zheng,Ling He,Tong Chen

Journal of oncology 2022:6093216 PubMed36618071

2022

Paeoniflorin Regulates NEDD4L/STAT3 Pathway to Induce Ferroptosis in Human Glioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hu Nie,Sheng Qiu,Ying Xing,Jie Xu,Bin Lu,Shu-Fa Zhao,Yun-Tao Li,Zhong-Zhou Su

Molecular cell 82:4277-4289.e10 PubMed36283413

2022

ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Benjamin M Adams,Vincent Chu,Joao A Paulo,Sichen Shao

Disease markers 2022:6967573 PubMed36193504

2022

miR-602 Activates NRF2 Antioxidant Pathways to Protect HBMECs from OGD/R-Induced Oxidative Stress via Targeting KEAP1 and HRD1.

Applications

Unspecified application

Species

Unspecified reactive species

Chunli Ma,Lei Yang,Qiang Gao,Lihua Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SYVN1/HRD1 antibody [EP7459]

Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (25)

Product promise