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AB249578

Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal SYVN1/HRD1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HRD1, KIAA1810, SYVN1, E3 ubiquitin-protein ligase synoviolin, RING-type E3 ubiquitin transferase synoviolin, Synovial apoptosis inhibitor 1

4 Images
Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)

This data was developed using ab170901, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)

This data was developed using ab170901, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)
  • IP

Supplier Data

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)

This data was developed using ab170901, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (<a href='/en-us/products/primary-antibodies/syvn1-hrd1-antibody-ep7459-ab170901'>ab170901</a>)

Predicted band size: 67 kDa

true

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)
  • WB

Supplier Data

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (AB249578)

This data was developed using ab170901, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (<a href='/en-us/products/primary-antibodies/syvn1-hrd1-antibody-ep7459-ab170901'>ab170901</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 67 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7459

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249578 is the carrier-free version of ab170901.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed : 12459480, PubMed : 12646171, PubMed : 12975321, PubMed : 14593114, PubMed : 16289116, PubMed : 16847254, PubMed : 17059562, PubMed : 17141218, PubMed : 17170702, PubMed : 22607976, PubMed : 26471130, PubMed : 28827405). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 12459480, PubMed : 12646171, PubMed : 12975321, PubMed : 14593114, PubMed : 16289116, PubMed : 16847254, PubMed : 17059562, PubMed : 17141218, PubMed : 17170702, PubMed : 22607976, PubMed : 26471130, PubMed : 28842558). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed : 17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed : 17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (By similarity). During the early stage of B cell development, required for degradation of the pre-B cell receptor (pre-BCR) complex, hence supporting further differentiation into mature B cells (By similarity).
See full target information SYVN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
View all publications
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Product promise

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