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Rabbit Recombinant Monoclonal T-bet / Tbx21 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (AB307194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (AB307194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (AB307194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (AB307194), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (AB307194), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Tested
Rat
Expected
Expected
Tested
Tested

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702). Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity). Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells (PubMed:33296702).

Alternative names

TBET, TBLYM, TBX21, T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM

Recommended products

Rabbit Recombinant Monoclonal T-bet / Tbx21 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27094-16
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

T-bet also known as Tbx21 is a T-box transcription factor that plays an important role in regulating immune responses. T-bet has a molecular mass of approximately 58 kDa. It is extensively expressed in T-helper 1 (Th1) cells and natural killer (NK) cells facilitating these cells' development and function. T-bet guides the transcriptional activities necessary for cellular differentiation and immune response by binding to specific DNA sequences. Its expression is tightly controlled reflecting its central role in immune regulation. "9d bet" is also referenced in scientific research although its specific context requires precise understanding within a given study.

Biological function summary

This transcription factor is pivotal for manufacturing interferon-gamma (IFN-γ) initiating and sustaining Th1 cell immunity. T-bet doesn't act alone; it functions as part of larger protein complexes interacting with other transcription factors and cofactors to exert its effects. The capacity to influence IFN-γ production makes T-bet a significant player in immune responses helping coordinate responses against intracellular pathogens. Moreover the expression of T-bet is not limited solely to T cells but also impacts other immune cells like CD8+ T cells and B cells. Anti-bet or anti-T antibodies frequently target T-bet in research to explore immune system intricacies better.

Pathways

T-bet integrates into the immune signaling network through pathways such as the JAK-STAT pathway and the Th1 differentiation pathway. It activates transcription of the IFNG gene working closely with related proteins like STAT4. T-bet's influence extends as it cooperates with STAT1 enabling a feed-forward loop that amplifies and stabilizes Th1 responses. "Bet t products" could refer to those derived from such transcriptional activation. Additionally T-bet involvement goes beyond transcriptional regulation affecting cytokine transport and secretion processes vital for an effective immune response.

Associated diseases and disorders

Researchers have linked T-bet to autoimmune diseases and infectious diseases. Aberrant expression of T-bet can lead to enhanced Th1 responses intensifying autoimmune conditions such as multiple sclerosis (MS). Its role in diseases connects it with NF-κB pathways and other transcription factors influencing inflammatory processes. Furthermore "anti bet" studies often involve understanding T-bet's role in infectious diseases seeking to manipulate its function to adjust immune responses in chronic infections. Studies targeting its pathways or interactions with disease-specific proteins aim to develop therapeutic strategies turning an element of dysregulation into a novel treatment approach.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on human cerebrum. The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse Burkitt's lymphoma tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse Burkitt's lymphoma. The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on rat spleen. The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 minss

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse spleen. The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human non-Hodgkin's lymphoma (T cell). The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human tonsil. The section was incubated with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using 307193, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    The lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] (Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193) at 1/1000 dilution

    All lanes: Mouse spleen tissue lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes cells labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 dilution (0.1µg)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Live cells were stained with anti-CD3 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193.

  • Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 dilution (0.1µg)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: CD19+ population. Live cells were stained with anti-CD19 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193.

  • Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using 307193, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: The lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the bands below 50 kDa are unknown.
    103 seconds
    Exposure time:

    All lanes: Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] (Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193) at 1/1000 dilution

    All lanes: Rat spleen tissue lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 103s

  • Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using 307193, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: K-562

    In lane 1, the lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the bands below 50 kDa are unknown.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-T-bet / Tbx21 antibody [EPR27094-16] (Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193) at 1/1000 dilution

    Lane 1: No-GFP-CD16.NK-92 (human malignant non-Hodgkins lymphoma natural killer cell) whole cell lysate 20 μg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer (NK-92®) cell with no GFP tag) cells labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 (1.044 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing nuclear staining in No-GFP-CD16.NK-92 cell line. Negative control: K562. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2µg/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse peripheral blood mononuclear cell (PBMC) cells labeling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 dilution (0.1µg)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: CD19+ population. Live cells were stained with anti-CD19 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193.

  • Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K562 (human chronic myelogenous leukemia lymphoblast, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag, Right) cells labelling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: K562.

  • Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-T-bet / Tbx21 antibody [EPR27094-16] - BSA and Azide free (ab307194)

    This data was developed using Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling T-bet / Tbx21 with Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193 at 1/500 dilution (0.1µg)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Live cells were stained with anti-CD56 conjugated to BV421. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or Anti-T-bet / Tbx21 antibody [EPR27094-16] ab307193.

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