JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB222290

Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal T-bet / Tbx21 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TBET, TBLYM, TBX21, T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free (AB222290)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free (AB222290)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin lymphoma tissue sections labeling T-bet / Tbx21 with Purified ab154200 at 1 : 500 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154200).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free (AB222290)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free (AB222290)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma labeling T-bet/Tbx21 with ab154200 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154200).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9302

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab222290 is the carrier-free version of ab154200.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

T-bet also known as Tbx21 is a T-box transcription factor that plays an important role in regulating immune responses. T-bet has a molecular mass of approximately 58 kDa. It is extensively expressed in T-helper 1 (Th1) cells and natural killer (NK) cells facilitating these cells' development and function. T-bet guides the transcriptional activities necessary for cellular differentiation and immune response by binding to specific DNA sequences. Its expression is tightly controlled reflecting its central role in immune regulation. "9d bet" is also referenced in scientific research although its specific context requires precise understanding within a given study.
Biological function summary

This transcription factor is pivotal for manufacturing interferon-gamma (IFN-γ) initiating and sustaining Th1 cell immunity. T-bet doesn't act alone; it functions as part of larger protein complexes interacting with other transcription factors and cofactors to exert its effects. The capacity to influence IFN-γ production makes T-bet a significant player in immune responses helping coordinate responses against intracellular pathogens. Moreover the expression of T-bet is not limited solely to T cells but also impacts other immune cells like CD8+ T cells and B cells. Anti-bet or anti-T antibodies frequently target T-bet in research to explore immune system intricacies better.

Pathways

T-bet integrates into the immune signaling network through pathways such as the JAK-STAT pathway and the Th1 differentiation pathway. It activates transcription of the IFNG gene working closely with related proteins like STAT4. T-bet's influence extends as it cooperates with STAT1 enabling a feed-forward loop that amplifies and stabilizes Th1 responses. "Bet t products" could refer to those derived from such transcriptional activation. Additionally T-bet involvement goes beyond transcriptional regulation affecting cytokine transport and secretion processes vital for an effective immune response.

Researchers have linked T-bet to autoimmune diseases and infectious diseases. Aberrant expression of T-bet can lead to enhanced Th1 responses intensifying autoimmune conditions such as multiple sclerosis (MS). Its role in diseases connects it with NF-κB pathways and other transcription factors influencing inflammatory processes. Furthermore "anti bet" studies often involve understanding T-bet's role in infectious diseases seeking to manipulate its function to adjust immune responses in chronic infections. Studies targeting its pathways or interactions with disease-specific proteins aim to develop therapeutic strategies turning an element of dysregulation into a novel treatment approach.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed : 10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed : 33296702). Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed : 27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity). Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells (PubMed : 33296702).
See full target information TBX21
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:647763 PubMed34055778

2021

EPC-Derived Exosomal miR-1246 and miR-1290 Regulate Phenotypic Changes of Fibroblasts to Endothelial Cells to Exert Protective Effects on Myocardial Infarction by Targeting ELF5 and SP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yulang Huang,Lifang Chen,Zongming Feng,Weixin Chen,Shaodi Yan,Rongfeng Yang,Jian Xiao,Jiajia Gao,Debao Zhang,Xiao Ke
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB154058

Anti-T-bet / Tbx21 antibody

Western blot - Anti-T-bet / Tbx21 antibody (AB154058)

  • 5
  • 1
Primary Antibodies

AB181400

Anti-T-bet / Tbx21 antibody - C-terminal

Western blot - Anti-T-bet / Tbx21 antibody - C-terminal (AB181400)

  • 0
  • 0
Primary Antibodies

AB91103

Anti-T-bet / Tbx21 antibody [39D, 3-9D]

Immunocytochemistry - Anti-T-bet / Tbx21 antibody [39D, 3-9D] (AB91103)

  • 0
  • 0
Primary Antibodies

AB150440

Anti-T-bet / Tbx21 antibody [EPR9301]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (AB150440)

  • 5
  • 1
Primary Antibodies

AB227115

Anti-T-bet / Tbx21 antibody [EPR9301] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] - BSA and Azide free (AB227115)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB199428

Anti-GATA3 antibody [EPR16651] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (AB199428)

  • 5
  • 13
Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587)

  • 5
  • 9
Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

BOND RX™ Validated|Recombinant|Lab Essentials|Advanced Validation

Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] (AB20034)

  • 4
  • 38
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com