Anti-T Plastin/PLS3 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (AB137585)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling T-Plastin protein using ab137585 at a 1/100 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T Plastin/PLS3 antibody (AB137585)

Immunohistochemical analysis of paraffin-embedded mouse cervix tissue stained for T-Plastin protein with ab137585 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.

• WB

Supplier Data

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 71 kDa

false

• WB

Supplier Data

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution

Lane 1:

293T whole cell extract at 30 µg

Lane 2:

A431 whole cell extract at 30 µg

Lane 3:

Jurkat whole cell extract at 30 µg

Lane 4:

Raji whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 71 kDa

false

• WB

Supplier Data

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/4000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

shRNA transfected 293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 71 kDa

false

• WB

Unknown

Western blot - Anti-T Plastin/PLS3 antibody (AB137585)

7.5% SDS PAGE

All lanes:

Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL-1 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false