Rabbit Polyclonal T Plastin/PLS3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLS3 aa 50-400.
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- Nature communications 14:9992023Tyrosyl-tRNA synthetase has a noncanonical function in actin bundling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiljana Ermanoska,Bob Asselbergh,Laura Morant,Maria-Luise Petrovic-Erfurth,Seyyedmohsen Hosseinibarkooie,Ricardo Leitão-Gonçalves,Leonardo Almeida-Souza,Sven Bervoets,Litao Sun,LaTasha Lee,Derek Atkinson,Akram Khanghahi,Ivaylo Tournev,Patrick Callaerts,Patrik Verstreken,Xiang-Lei Yang,Brunhilde Wirth,Avital A Rodal,Vincent Timmerman,Bruce L Goode,Tanja A Godenschwege,Albena JordanovaPubMed 36890170
- Endocrinology 160:484-5032019Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Wen et. al.PubMed 30649248
- Cell death & disease 9:2082018Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis.
- Scientific reports 7:11102017Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2.
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31:584-5972016Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Gao,Dolores Mruk,Haiqi Chen,Wing-Yee Lui,Will M Lee,C Yan ChengPubMed 27815338
- Scientific reports 6:285892016Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Gao et. al.PubMed 27358069
- Spermatogenesis 6:e12063532016Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Li et. al.PubMed 27559491
- Gastroenterology 146:1386-96.e1-172014Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link P13797
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Alternative names
Plastin-3, T-fimbrin, T-plastin, PLS3
Recommended products
Rabbit Polyclonal T Plastin/PLS3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLS3 aa 50-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link P13797
- Purification technique
- Affinity purification Immunogen
- Specificity
ab137585 may also detect Plastin L as the sequence shows 82% identity.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The T Plastin also known as PLS3 is a protein with a mass of around 70 kDa. This protein belongs to the plastin family and another popular alias is plastine. T Plastin participates in cellular mechanics by bundling actin filaments which is essential for maintaining cell structure and motility. It occurs in various tissues but is highly expressed in the intestines testis and other cells with rapid movement needs like immune cells. Its presence supports functions needing dynamic rearrangement of the cytoskeleton.
- Biological function summary
T Plastin plays significant roles in several cellular activities. It stabilizes the actin cytoskeleton which impacts cell division signal transduction and cellular interactions. T Plastin is not part of a multi-protein complex but works closely with actin and other actin-binding proteins to regulate cytoskeletal organization. By supporting these functions T Plastin indirectly influences cell shape and intracellular transport mechanisms.
- Pathways
T Plastin integrates into the actin dynamics pathways important for cytoskeletal reorganization. It works alongside proteins such as cofilin and profilin which also regulate actin dynamics. Another important pathway includes wound healing where T Plastin's actin-bundling function assists in cell migration and tissue regeneration. In these pathways its role ensures efficient cellular responses to environmental changes.
- Associated diseases and disorders
Defects in T Plastin have been linked to osteoporosis and some forms of cancers. In osteoporosis improper expression of T Plastin affects bone density regulation partly due to its influence on osteoclast function. Cancers such as colorectal cancer exhibit altered T Plastin levels which may impact metastasis due to changes in cell motility. In these contexts T Plastin's interaction with other proteins like integrins highlights its importance in controlling cell adhesion and migration associated with disease progress.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-T Plastin/PLS3 antibody (ab137585)
7.5% SDS PAGE
All lanes: Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/500 dilution
Lane 1: HeLa S3 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 50 kDa, 60 kDa, 71 kDa
Western blot - Anti-T Plastin/PLS3 antibody (ab137585)
7.5% SDS PAGE
All lanes: Western blot - Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution
Lane 1: NIH-3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Predicted band size: 71 kDa
Immunocytochemistry/ Immunofluorescence - Anti-T Plastin/PLS3 antibody (ab137585)
Immunofluorescence analysis of methanol-fixed HeLa cells labelling T Plastin/PLS3 with ab137585 at 1/200 dilution.
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