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AB229015

Anti-T1R3 antibody

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(3 Publications)

Rabbit Polyclonal T1R3 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TAS1R3.

View Alternative Names

T1R3, TR3, TAS1R3, Taste receptor type 1 member 3, Sweet taste receptor T1R3

1 Images
Western blot - Anti-T1R3 antibody (AB229015)
  • WB

Supplier Data

Western blot - Anti-T1R3 antibody (AB229015)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-T1R3 antibody (ab229015) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract at 30 µg

Lane 2:

K562 membrane extract at 30 µg

Predicted band size: 93 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TAS1R3. The exact immunogen used to generate this antibody is proprietary information.

Q7RTX0

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Alternative Products

Primary Antibodies

AB150525

Anti-T1R3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T1R3 antibody (AB150525)

  • 4
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Complementary Products

Secondary Antibodies

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Primary Antibodies

AB230788

Anti-TR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR1 antibody (AB230788)

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Primary Antibodies

AB77346

Anti-GPCR TAS1R2 antibody

Western blot - Anti-GPCR TAS1R2 antibody (AB77346)

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Primary Antibodies

AB222859

Anti-TAS2R38 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAS2R38 antibody (AB222859)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

T1R3 also known as TAS1R3 functions as a taste receptor. Weighing around 90kDa T1R3 is part of the G protein-coupled receptor family. It is largely expressed in the taste buds on the tongue and some extraoral tissues. T1R3 partners with TAS1R1 or TAS1R2 to detect umami and sweet taste respectively. This protein is critical in the detection and signaling of taste perceptions in the gustatory pathways.
Biological function summary

T1R3 plays a role in nutrient sensing and metabolic regulation. As part of a receptor complex T1R3 partners with TAS1R2 to form a sweet taste receptor and pairs with TAS1R1 to form an umami receptor. This pairing occurs at the cell surface in the taste buds and is vital for the recognition of specific ligands like amino acids and sugars. T1R3's ability to sense nutrients impacts digestive processes and energy balance.

Pathways

T1R3 involvement extends to signaling pathways related to taste signaling and energy homeostasis. T1R3 activates taste GPCR pathways which contribute to neurotransmitter release in taste perceptions. It connects to the phosphatidylinositol signaling system pathway impacting cellular processes through interaction with proteins like PLCβ2. T1R3 also interfaces with cellular mechanisms influenced by serotonin and glutamate receptors.

T1R3 has a connection to metabolic diseases including obesity and diabetes. It influences glucose metabolism and energy intake linking it to these conditions. T1R3 has interactions with other proteins such as leptin which is involved in body weight regulation. Impairment or alterations in T1R3 function can correlate with dietary-related disorders impacting disease progression through metabolic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
See full target information TAS1R3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Medicine 102:e33908 PubMed37266606

2023

Involvement of NY-ESO-1 and MAGE-A4 in the pathogenesis of desmoid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiko Hashimoto,Shunji Nishimura,Yu Shinyashiki,Tomohiko Ito,Ryosuke Kakinoki,Masao Akagi

Clinical and translational medicine 12:e1021 PubMed35988262

2022

Sweet taste receptor agonists attenuate macrophage IL-1β expression and eosinophilic inflammation linked to autophagy deficiency in myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinju Lee,So Jeong Kim,Go Eun Choi,Eunbi Yi,Hyo Jin Park,Woo Seon Choi,Yong Ju Jang,Hun Sik Kim
View all publications
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Product promise

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