AB9115

Anti-T7 tag® antibody

Name: Anti-T7 tag® antibody (ab9115) | Abcam
Brand: Abcam Limited
SKU: AB9115
Price: 525 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal T7 tag® antibody. Suitable for I-ELISA, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 9 publications.

View Alternative Names

Minor capsid protein, Gene product 10B, Minor head protein, Gp10B, 10

1 Images

Indirect ELISA - Anti-T7 tag® antibody (AB9115)

I-ELISA

Supplier Data

Indirect ELISA - Anti-T7 tag® antibody (AB9115)

Antigen concentration : 500 ng/well. Secondary : HRP Goat anti-Rabbit lgG (Pre-adsorbed). Developed with TMB (10 minutes). Microplate reader set at 450nm.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Applications

ELISA, ICC/IF, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

T7-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Assembles with the major capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins (PubMed : 20962334). The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid formed, encapsidates one single copy of the viral genome.

See full target information 10

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:1711-1732 PubMed36469690

2022

Substitution of PINK1 Gly411 modulates substrate receptivity and turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Fabienne C Fiesel,Dominika Fričová,Caleb S Hayes,Mathew A Coban,Roman Hudec,Jenny M Bredenberg,Benjamin J Broadway,Briana N Markham,Tingxiang Yan,Paige K Boneski,Gabriella Fiorino,Jens O Watzlawik,Xu Hou,Arthur M McCarty,Laura J Lewis-Tuffin,Jun Zhong,Benjamin J Madden,Alban Ordureau,Heeseon An,Andreas Puschmann,Zbigniew K Wszolek,Owen A Ross,J Wade Harper,Thomas R Caulfield,Wolfdieter Springer

Advanced biology 5:e2000176 PubMed33724733

2021

Enhancement of Wound Healing Efficacy by Increasing the Stability and Skin-Penetrating Property of bFGF Using 30Kc19α-Based Fusion Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Haein Lee,Young-Hyeon An,Tae Keun Kim,Jina Ryu,G Kate Park,Mihn Jeong Park,Junghyeon Ko,Hyunbum Kim,Hak Soo Choi,Nathaniel S Hwang,Tai Hyun Park

Journal of integrative plant biology 62:1552-1573 PubMed32129570

2020

A bHLH transcription activator regulates defense signaling by nucleo-cytosolic trafficking in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Fanwei Meng,Chao Yang,Jidong Cao,Huan Chen,Jinhuan Pang,Qiqi Zhao,Zongyi Wang,Zheng Qing Fu,Jun Liu

Molecular biology of the cell 29:2470-2480 PubMed30091641

2018

WDR62 mediates TNFα-dependent JNK activation via TRAF2-MLK3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Elad Prinz,Sharon Aviram,Ami Aronheim

Oncotarget 9:22945-22959 PubMed29796164

2018

Using antibody directed phototherapy to target oesophageal adenocarcinoma with heterogeneous HER2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley Pye,Mohammed Adil Butt,Laura Funnell,Halla W Reinert,Ignazio Puccio,Saif U Rehman Khan,Savvas Saouros,Jared S Marklew,Ioanna Stamati,Maryam Qurashi,Rehan Haidry,Vinay Sehgal,Dahmane Oukrif,Michael Gandy,Hayley C Whitaker,Manuel Rodriguez-Justo,Marco Novelli,Rifat Hamoudi,Gokhan Yahioglu,Mahendra P Deonarain,Laurence B Lovat

Mobile DNA 9:11 PubMed29610582

2018

The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Herrmann,Sabine Wittmann,Dominique Thomas,Caitlin N Shepard,Baek Kim,Nerea Ferreirós,Thomas Gramberg

Molecular neurodegeneration 12:32 PubMed28438176

2017

The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Ando,Fabienne C Fiesel,Roman Hudec,Thomas R Caulfield,Kotaro Ogaki,Paulina Górka-Skoczylas,Dariusz Koziorowski,Andrzej Friedman,Li Chen,Valina L Dawson,Ted M Dawson,Guojun Bu,Owen A Ross,Zbigniew K Wszolek,Wolfdieter Springer

Nucleic acids research 42:396-416 PubMed24101588

2013

Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity.

Applications

ICC/IF

Species

Human

Axel V Horn,Sabine Klawitter,Ulrike Held,André Berger,Ananda Ayyappan Jaguva Vasudevan,Anja Bock,Henning Hofmann,Kay-Martin O Hanschmann,Jan-Hendrik Trösemeier,Egbert Flory,Robert A Jabulowsky,Jeffrey S Han,Johannes Löwer,Roswitha Löwer,Carsten Münk,Gerald G Schumann

The Journal of biological chemistry 283:25660-70 PubMed18635538

2008

Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression.

Applications

Species

Unspecified reactive species

Toru Kubota,Mayumi Matsuoka,Tsung-Hsien Chang,Prafullakumar Tailor,Tsuguo Sasaki,Masato Tashiro,Atsushi Kato,Keiko Ozato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-T7 tag® antibody

Indirect ELISA - Anti-T7 tag® antibody (AB9115)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (9)

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