Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free

9 product images

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  Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-T7 tag® antibody [EPR28033-8] (Anti-T7 tag® antibody [EPR28033-8] ab317258) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an Human Histone H3.3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST

  Lane 2: 293T (human embryonic kidney epithelial cell) transfected with an Human Histone H3 expression vector containing a T7 tag®, whole cell lysate at 20 µg with NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 15 kDa

  Exposure time: 55s

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  Indirect ELISA - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Indirect ELISA analysis of Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

  Antigen: T7 tag®.

  Antigen concentration: 1000 ng/ml

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  Immunocytochemistry/ Immunofluorescence - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) transfected with a T7 tag®-CTCF expression vector cells labelling T7 tag® with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/1000 (0.527 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing nuclear staining in HeLa cells transfected with a T7 tag®-CTCF expression vector (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunoprecipitation - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  T7 tag® was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysate with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag®, whole cell lysate
  
  Lane 2: Anti-T7 tag® antibody [EPR28033-8] ab317258 IP in HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-T7 tag® antibody [EPR28033-8] ab317258 in HeLa transfected with an Human CTCF expression vector containing a T7 tag® whole cell lysate

  All lanes: Immunoprecipitation - Anti-T7 tag® antibody [EPR28033-8] (Anti-T7 tag® antibody [EPR28033-8] ab317258) at 1/30 dilution

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell) transfected with an Human CTCF expression vector containing a T7 tag®, whole cell lysate with NFDM/TBST

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 41s

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  Western blot - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  In Western blot, Anti-CTCF antibody [EPR7314 (B)] - ChIP Grade (Anti-CTCF antibody [EPR7314(B)] - ChIP Grade ab128873) staining at 1/1000 dilution.

  All lanes: Western blot - Anti-T7 tag® antibody [EPR28033-8] (Anti-T7 tag® antibody [EPR28033-8] ab317258) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with empty vector, whole cell lysate at 20 µg with NFDM/TBST

  Lane 2: HeLa transfected with an Human CTCF expression vector containi a T7 tag®, whole cell lysate at 20 µg with NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 150 kDa, 36 kDa

  Exposure time: 8s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling T7 tag® with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat cerebrum.The section was incubated with Anti-T7 tag® antibody [EPR28033-8] ab317258 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling T7 tag® with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse cerebrum.The section was incubated with Anti-T7 tag® antibody [EPR28033-8] ab317258 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling T7 tag® with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on human cerebrum.The section was incubated with Anti-T7 tag® antibody [EPR28033-8] ab317258 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T7 tag® antibody [EPR28033-8] - BSA and Azide free (ab317259)

  This data was developed using Anti-T7 tag® antibody [EPR28033-8] ab317258, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded (A) Hela (human cervix adenocarcinoma epithelial cell) transfected with a T7 tag®-CTCF expression vector cell pellet. (B) Hela transfected with empty vector cell pellet tissue labeling T7 tag® with Anti-T7 tag® antibody [EPR28033-8] ab317258 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on Hela transfected with a T7 tag®-CTCF expression vector cell pellet (image A). No staining on Hela transfected with empty vector cell pellet (image B).The section was incubated with Anti-T7 tag® antibody [EPR28033-8] ab317258 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins