Rabbit Polyclonal TAB1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TAB1 aa 450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TAB1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15750
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Key adapter protein that plays an essential role in JNK and NF-kappa-B activation and proinflammatory cytokines production in response to stimulation with TLRs and cytokines (PubMed:22307082, PubMed:24403530). Mechanistically, associates with the catalytic domain of MAP3K7/TAK1 to trigger MAP3K7/TAK1 autophosphorylation leading to its full activation (PubMed:10838074, PubMed:25260751, PubMed:37832545). Similarly, associates with MAPK14 and triggers its autophosphorylation and subsequent activation (PubMed:11847341, PubMed:29229647). In turn, MAPK14 phosphorylates TAB1 and inhibits MAP3K7/TAK1 activation in a feedback control mechanism (PubMed:14592977). Plays also a role in recruiting MAPK14 to the TAK1 complex for the phosphorylation of the TAB2 and TAB3 regulatory subunits (PubMed:18021073).
Alternative names
MAP3K7IP1, TAB1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TGF-beta-activated kinase 1-binding protein 1, TAK1-binding protein 1
Recommended products
Rabbit Polyclonal TAB1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TAB1 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TAB1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15750
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245587 was affinity purified using an epitope specific to TAB1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAB1 also known as TGF-beta-activated kinase 1 binding protein 1 is a protein involved in signal transduction processes. It has a mass of approximately 68 kDa. TAB1 is expressed in various human tissues with notable presence in immune cells such as macrophages and lymphocytes. Mechanically TAB1 acts as a regulatory molecule that binds to and activates TAK1 an important kinase involved in several signaling cascades. This interaction facilitates the phosphorylation events necessary for downstream signal propagation.
- Biological function summary
TAB1 functions as a scaffold protein assisting in the assembly of signaling complexes. This protein is part of the TAK1 complex which also includes TAB2 and TAB3. The formation of this complex is essential for activating multiple intracellular signaling pathways. Through its role in the TAK1 complex TAB1 contributes to cellular responses to environmental cues including inflammation apoptosis and immune responses. Its activity influences transcription factor activation and is pivotal in orchestrating cellular adaptation to changes.
- Pathways
TAB1 is essential in both the NF-kB and MAPK signaling pathways. Maintaining proper function in these pathways is important for inflammatory responses and stress-related signal transduction. TAB1 helps bridge the activation of TAK1 to the downstream signaling required for NF-kB pathway activation. Additionally in the MAPK pathway TAB1 influences the activation of proteins such as MKKs which further regulate cellular stress responses and developmental processes.
- Associated diseases and disorders
TAB1 plays a role in inflammatory diseases and cancer. Dysregulation of TAB1 activity can result in chronic inflammation contributing to conditions like rheumatoid arthritis. In cancer altered TAB1 expression or function can lead to unchecked cell growth and survival. TAB1 connects to other proteins such as TAK1 in these diseases as it modulates signaling pathways that determine cellular fate. Understanding TAB1's involvement in these disorders opens potential therapeutic avenues targeting its interactions and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TAB1 antibody (ab245587)
All lanes: Western blot - Anti-TAB1 antibody (ab245587) at 0.1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 55 kDa
Exposure time: 3min
Immunoprecipitation - Anti-TAB1 antibody (ab245587)
TAB1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245587 used for IP at 3 μg/mg lysate. For WB 1 μg/ml.
Lane 1: ab245587 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
All lanes: Immunoprecipitation - Anti-TAB1 antibody (ab245587)
Predicted band size: 55 kDa
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