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Rabbit Recombinant Monoclonal TAB1 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-TAB1 antibody [EPR29000-94] (AB324014), expandable thumbnail
  • Western blot - Anti-TAB1 antibody [EPR29000-94] (AB324014), expandable thumbnail
  • Western blot - Anti-TAB1 antibody [EPR29000-94] (AB324014), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Key adapter protein that plays an essential role in JNK and NF-kappa-B activation and proinflammatory cytokines production in response to stimulation with TLRs and cytokines (PubMed:22307082, PubMed:24403530). Mechanistically, associates with the catalytic domain of MAP3K7/TAK1 to trigger MAP3K7/TAK1 autophosphorylation leading to its full activation (PubMed:10838074, PubMed:25260751, PubMed:37832545). Similarly, associates with MAPK14 and triggers its autophosphorylation and subsequent activation (PubMed:11847341, PubMed:29229647). In turn, MAPK14 phosphorylates TAB1 and inhibits MAP3K7/TAK1 activation in a feedback control mechanism (PubMed:14592977). Plays also a role in recruiting MAPK14 to the TAK1 complex for the phosphorylation of the TAB2 and TAB3 regulatory subunits (PubMed:18021073).

Alternative names

MAP3K7IP1, TAB1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TGF-beta-activated kinase 1-binding protein 1, TAK1-binding protein 1

Rabbit Recombinant Monoclonal TAB1 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29000-94
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014), expandable thumbnail

    Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014)

    TAB1 Western blot staining using rabbit Anti-TAB1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 5: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 6: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 7: Mouse thymus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014), expandable thumbnail

    Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014)

    TAB1 Western blot staining using rabbit Anti-TAB1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa, 36 kDa

    Exposure time: 125s

  • Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014), expandable thumbnail

    Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014)

    TAB1 Western blot staining using rabbit Anti-TAB1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Performed under reducing conditions.

    In Western blot, ab324014 was shown to bind specifically to TAB1. Target of interest was observed at 55 kDa in wild-type HEK293T cell lysate (Lane 1) with no signal observed at this size in TAB1 knockout cell line (Lane 2) (Lane 2, knockout cell line Human TAB1 knockout HEK-293T cell line ab266845/ knockout cell lysate Human TAB1 knockout HEK-293T cell lysate ab258220).

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TAB1 antibody [EPR29000-94] (ab324014) at 1/1000 dilution

    Lane 1: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: Western blot - Human TAB1 knockout HEK-293T cell lysate (Human TAB1 knockout HEK-293T cell lysate ab258220) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 55 kDa, 36 kDa

    Exposure time: 92s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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