Skip to main content

Rabbit Recombinant Monoclonal TAB2 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (AB317602), expandable thumbnail
  • Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602), expandable thumbnail
  • Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602), expandable thumbnail
  • Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (PubMed:10882101, PubMed:11460167, PubMed:15327770, PubMed:22158122). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (PubMed:10882101, PubMed:11460167, PubMed:15327770, PubMed:22158122). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed:15327770, PubMed:22158122). Regulates the IL1-mediated translocation of NCOR1 out of the nucleus (By similarity). Involved in heart development (PubMed:20493459).

Alternative names

TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TGF-beta-activated kinase 1-binding protein 2, TAB-2, MAP3K7IP2, KIAA0733, TAB2

Recommended products

Rabbit Recombinant Monoclonal TAB2 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Alternative names

TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TGF-beta-activated kinase 1-binding protein 2, TAB-2, MAP3K7IP2, KIAA0733, TAB2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19670-161

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TAB2 also known as TGF-beta-activated kinase 1 binding protein 2 or MAP3K7IP2 is an adapter protein with a mass of approximately 71 kDa. It plays a mechanical role in the nuclear and cytoplasmic compartments acting as a critical component in signaling pathways. Its expression is notable in various tissues including the heart placenta and skeletal muscle. TAB2 mainly functions by interacting with kinases to relay and amplify signals in the cell especially those related to inflammation and stress responses.

Biological function summary

TAB2 maintains a significant position in cellular signaling as part of larger protein complexes. It associates with TAK1 (transforming growth factor-beta-activated kinase 1) to regulate NF-kappaB and MAPK pathways. Through these interactions TAB2 helps cells respond to environmental signals mediating transcriptional responses necessary for adaptation and defense mechanisms. In particular TAB2's role in these complexes underlines its importance in immune response modulation and cellular homeostasis.

Pathways

TAB2 is deeply involved in both the NF-kappaB and MAPK signaling pathways where it cooperates intimately with proteins such as TAK1 and TAB1. These pathways are essential for gene expression regulation in response to pro-inflammatory signals. TAB2 facilitates the activation of IKK complex within the NF-kappaB pathway and JNK in the MAPK pathway. By influencing these pathways TAB2 governs important cellular processes like apoptosis proliferation and differentiation.

Associated diseases and disorders

TAB2's dysfunction has strong connections with inflammatory and cardiovascular diseases. Its interaction with TAK1 and other proteins can lead to aberrant signaling therefore associating TAB2 with conditions such as rheumatoid arthritis and cardiac hypertrophy. In cardiomyopathies for instance disturbances in TAB2's function can affect signal transduction significantly impacting cardiac muscle structure and function. Understanding TAB2 and its intricate network of protein interactions aids in uncovering therapeutic targets for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602), expandable thumbnail

    Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602)

    TAB2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317602 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317602 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab317602 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317602 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602), expandable thumbnail

    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the lower molecular weight bands are unknown.

    Exposure time: Lanes 1: 92 seconds; Lane 2: 114 seconds.

    All lanes: Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602), expandable thumbnail

    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)

    Low expression: Jurkat

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    The identity of the lower molecular weight bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4: 158 seconds; Lane 5-8: 180 seconds.

    All lanes: Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 5: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 6: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 7: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 8: NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa, 36 kDa

  • Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602), expandable thumbnail

    Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602)

    This antibody is specific to TAB2 and does not cross react with endogenous TAB3.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TAB3 antibody [EPR5965] (Anti-TAB3 antibody [EPR5965] ab124723) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting TAB2 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa, 36 kDa

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com