Anti-TAB2 antibody [EPR19670-161]

• IP

Supplier Data

Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (AB317602)

TAB2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317602 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317602 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab317602 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317602 in HeLa whole cell lysate

All lanes:

Immunoprecipitation - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602)

Western blot : Anti-TAB2 antibody [EPR19670-161] ab317602 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80 kDa in Wild-type A549 cell lysates with no signal observed at this size in TAB2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

Lane 1:

Wild-type A549 whole cell lysate at 20 µg

Lane 2:

Western blot - Human TAB2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tab2-knockout-a549-cell-line-ab301263'>ab301263</a>) at 20 µg

Lane 3:

HEK-293 whole cell lysate at 20 µg

Lane 4:

Raji whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 76 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602)

This antibody is specific to TAB2 and does not cross react with endogenous TAB3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-TAB3 antibody [EPR5965] (ab124723) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting TAB2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602)

Low expression : Jurkat

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

The identity of the lower molecular weight bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-4 : 158 seconds; Lane 5-8 : 180 seconds.

All lanes:

Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 5:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 6:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 7:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 8:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-TAB2 antibody [EPR19670-161] (AB317602)

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

The identity of the lower molecular weight bands are unknown.

Exposure time : Lanes 1 : 92 seconds; Lane 2 : 114 seconds.

All lanes:

Western blot - Anti-TAB2 antibody [EPR19670-161] (ab317602) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

true