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AB85655

Anti-TAB3 antibody

5

(3 Reviews)

|

(8 Publications)

Rabbit Polyclonal TAB3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TAB3 aa 600-650.

View Alternative Names

MAP3K7IP3, TAB3, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, Mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, TGF-beta-activated kinase 1-binding protein 3, TAB-3

3 Images
Immunoprecipitation - Anti-TAB3 antibody (AB85655)
  • IP

Unknown

Immunoprecipitation - Anti-TAB3 antibody (AB85655)

1mg whole cell lysate from HeLa cells was immunoprecipitated using 10μg ab85655.
20% of the immunoprecipitate was loaded in each lane, and probed with ab85655 at 1μg/ml (lane 1) or with a control IgG (lane 2).
Detection : chemoluminescence, with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TAB3 antibody (ab85655)

Predicted band size: 79 kDa

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Western blot - Anti-TAB3 antibody (AB85655)
  • WB

Unknown

Western blot - Anti-TAB3 antibody (AB85655)

All lanes:

Western blot - Anti-TAB3 antibody (ab85655) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 79 kDa

false

Exposure time: 30s

Western blot - Anti-TAB3 antibody (AB85655)
  • WB

CiteAb

Western blot - Anti-TAB3 antibody (AB85655)

TAB3 western blot using anti-TAB3 antibody ab85655. Publication image and figure legend from Zhao, N., Wang, R., et al., 2014, Mol Cancer, PubMed 24565101.

ab85655 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab85655 please see the product overview.

miR-26b suppresses NF-κB signaling by targeting TAK1 and TAB3. (A) Knockdown of TAK1 or TAB3 inhibited the TNFα-induced NF-κB reporter activity. QGY-7703 cells were treated and analyzed as in Figure 1A. (B) Knockdown of TAK1 or TAB3 attenuated the TNFα-induced phosphorylation of IκBα and p65. QGY-7703 cells transfected with siNC (lanes 1, 2), siTAK1 (lanes 3, 4) or siTAB3 (lanes 5, 6) were untreated (-) or treated with 20 ng/ml TNFα (+) for 3 minutes before immunoblot. (C) miR-26b repressed the activity of the luciferase reporter containing the wild-type 3'UTR of TAK1 or TAB3. QGY-7703 cells were co-transfected with NC or miR-26b duplexes, pRL-TK and a firefly luciferase reporter plasmid carrying the wild-type (WT) or the mutant (MUT) 3'UTR of TAK1 or TAB3 before luciferase activity analysis. (D) Expression of miR-26b reduced the protein levels of cellular TAK1 and TAB3. HCC cells were transfected with NC or miR-26b duplexes for 48 hours before immunoblot. (E) Antagonism of endogenous miR-26b enhanced the levels of TAK1 and TAB3 proteins. HCC cells were transfected with anti-NC or anti-miR-26b for 48 hours before immunoblot. *, P < 0.05; **, P < 0.01.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TAB3 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

Q8N5C8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAB3 also known as TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 is an essential protein that modulates important cellular processes. It has a molecular mass of approximately 90 kDa. The protein functions mechanically to activate downstream signaling pathways acting as an adapter molecule. Expressed ubiquitously TAB3 has more pronounced expression in tissues such as the liver suggesting its potential role in organ-specific functions.
Biological function summary

TAB3 influences multiple cellular signaling mechanisms contributing to the regulation of inflammatory responses and cell survival. It forms a part of important signaling complexes specifically the TAK1 complex which includes TAB1 and TAB2. Together this complex modulates pathways that are important for immune response and stress signaling in cells impacting the cell's ability to respond effectively to external signals.

Pathways

Research shows TAB3's involvement in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. As part of these pathways it interacts closely with other proteins including TAK1 orchestrating a coordinated cellular response to inflammatory and stress stimuli. The activity of these pathways is essential for maintaining proper cell signaling and gene expression under various physiological and pathological conditions.

Studies connect TAB3 to inflammatory and autoimmune diseases such as rheumatoid arthritis and inflammatory bowel disease. Its role alongside TAK1 and NF-κB in these conditions highlights its significance in managing inflammatory responses. Understanding TAB3’s interactions and regulation could offer insights into therapeutic targets for managing these autoimmune and inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122, PubMed : 36593296). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2 : this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed : 15327770, PubMed : 18079694, PubMed : 22158122).. Isoform 2. May be an oncogenic factor.
See full target information TAB3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 14: PubMed40990917

2025

Ubiquitination-activated TAB-TAK1-IKK-NF-κB axis modulates gene expression for cell survival in the lysosomal damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Akinori Endo,Chikage Takahashi,Naoko Ishibashi,Yasumasa Nishito,Koji Yamano,Keiji Tanaka,Yukiko Yoshida

American journal of translational research 13:12694-12703 PubMed34956484

2021

MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyu Zhang,Qingbo Lu,Haigang Pang,Min Zhang,Wenhai Wei

The FEBS journal 287:3165-3183 PubMed31903660

2020

USP15 potentiates NF-κB activation by differentially stabilizing TAB2 and TAB3.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoqiao Zhou,Cheng Cheng,Yujuan Wei,Jing Yang,Wanzhu Zhou,Qiuyi Song,Mengxiang Ke,Wanyao Yan,Ling Zheng,Yu Zhang,Kun Huang

Nature communications 9:4381 PubMed30348973

2018

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjie Sheng,Chen Yao,Ziyang Wang,Hongyuan Chen,Yu Zhao,Dazhi Xu,Haojie Huang,Wenlin Huang,Shuai Chen

Archives of medical science : AMS 17:500-513 PubMed33747285

2018

MicroRNA-16 inhibits the lipopolysaccharide-induced inflammatory response in nucleus pulposus cells of the intervertebral disc by targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Ketao Du,Xuguang He,Jiaqin Deng

FEBS letters 591:1141-1149 PubMed28295271

2017

NLRP6 facilitates the interaction between TAB2/3 and TRIM38 in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

WB

Species

Human

Yang Lin,Zhengqiang Luo

Molecular cancer 13:35 PubMed24565101

2014

MicroRNA-26b suppresses the NF-κB signaling and enhances the chemosensitivity of hepatocellular carcinoma cells by targeting TAK1 and TAB3.

Applications

WB

Species

Human

Na Zhao,Ruizhi Wang,Liangji Zhou,Ying Zhu,Jiao Gong,Shi-Mei Zhuang

The American journal of pathology 180:2293-308 PubMed22525462

2012

The critical role of TAK1 in accentuated epithelial to mesenchymal transition in obliterative bronchiolitis after lung transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron Gardner,Andrew J Fisher,Christine Richter,Gail E Johnson,Elizabeth J Moisey,Malcolm Brodlie,Christopher Ward,Anja Krippner-Heidenreich,Derek A Mann,Lee A Borthwick
View all publications
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