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AB124723

Anti-TAB3 antibody [EPR5965]

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(6 Publications)

Rabbit Recombinant Monoclonal TAB3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

MAP3K7IP3, TAB3, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, Mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, TGF-beta-activated kinase 1-binding protein 3, TAB-3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] (AB124723)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] (AB124723)

ab124723, at 1/50 dilution, staining TAB3 in HepG2 cells by Immunofluorescence.

Western blot - Anti-TAB3 antibody [EPR5965] (AB124723)
  • WB

Unknown

Western blot - Anti-TAB3 antibody [EPR5965] (AB124723)

All lanes:

Western blot - Anti-TAB3 antibody [EPR5965] (ab124723) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

Western blot - Anti-TAB3 antibody [EPR5965] (AB124723)
  • WB

Supplier Data

Western blot - Anti-TAB3 antibody [EPR5965] (AB124723)

This antibody is specific to TAB2 and does not cross react with endogenous TAB3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-TAB3 antibody [EPR5965] (ab124723) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TAB2 antibody [EPR19670-161] (<a href='/en-us/products/primary-antibodies/tab2-antibody-epr19670-161-ab317602'>ab317602</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

HeLa transfected with siRNA specifically targeting TAB2 whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

OI-RD Scanning - Anti-TAB3 antibody [EPR5965] (AB124723)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TAB3 antibody [EPR5965] (AB124723)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-TAB3 antibody [EPR5965] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5965

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAB3 also known as TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 is an essential protein that modulates important cellular processes. It has a molecular mass of approximately 90 kDa. The protein functions mechanically to activate downstream signaling pathways acting as an adapter molecule. Expressed ubiquitously TAB3 has more pronounced expression in tissues such as the liver suggesting its potential role in organ-specific functions.
Biological function summary

TAB3 influences multiple cellular signaling mechanisms contributing to the regulation of inflammatory responses and cell survival. It forms a part of important signaling complexes specifically the TAK1 complex which includes TAB1 and TAB2. Together this complex modulates pathways that are important for immune response and stress signaling in cells impacting the cell's ability to respond effectively to external signals.

Pathways

Research shows TAB3's involvement in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. As part of these pathways it interacts closely with other proteins including TAK1 orchestrating a coordinated cellular response to inflammatory and stress stimuli. The activity of these pathways is essential for maintaining proper cell signaling and gene expression under various physiological and pathological conditions.

Studies connect TAB3 to inflammatory and autoimmune diseases such as rheumatoid arthritis and inflammatory bowel disease. Its role alongside TAK1 and NF-κB in these conditions highlights its significance in managing inflammatory responses. Understanding TAB3’s interactions and regulation could offer insights into therapeutic targets for managing these autoimmune and inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122, PubMed : 36593296). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2 : this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed : 15327770, PubMed : 18079694, PubMed : 22158122).. Isoform 2. May be an oncogenic factor.
See full target information TAB3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301641 PubMed37587766

2023

USP25 Inhibits Neuroinflammatory Responses After Cerebral Ischemic Stroke by Deubiquitinating TAB2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongding Li,Baohua Liu,Kate Lykke Lambertsen,Bettina Hjelm Clausen,Zhenhu Zhu,Xue Du,Yanqi Xu,Frantz Rom Poulsen,Bo Halle,Christian Bonde,Meng Chen,Xue Wang,Dirk Schlüter,Jingyong Huang,Ari Waisman,Weihong Song,Xu Wang

Nature communications 14:944 PubMed36805456

2023

Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Haobo Han,Jiakai Xing,Wenqi Chen,Jiaxin Jia,Quanshun Li

Cell death and differentiation 29:1513-1527 PubMed35105963

2022

EBV infection-induced GPX4 promotes chemoresistance and tumor progression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yuan,Shibing Li,Qiuyan Chen,Tianliang Xia,Donghua Luo,Liangji Li,Sailan Liu,Shanshan Guo,Liting Liu,Chaochao Du,Guodong Jia,Xiaoyun Li,Zijian Lu,Zhenchong Yang,Huanliang Liu,Haiqiang Mai,Linquan Tang

Molecular medicine reports 18:5799-5806 PubMed30365083

2018

Downregulation of microRNA‑30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Liu,Tingshu Jiang,Xingang Hu,Zhida Liu,Liming Zhao,Hongmei Liu,Zhaihua Liu,Lijun Ma

PloS one 11:e0152721 PubMed27023403

2016

Hepatitis B Virus Stimulated Fibronectin Facilitates Viral Maintenance and Replication through Two Distinct Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Ren,Jun Wang,Tie-Long Chen,Hao-Yu Li,Yu-Shun Wan,Nan-Fang Peng,Xi-En Gui,Ying Zhu

Proceedings of the National Academy of Sciences of 111:1509-14 PubMed24434549

2014

TRIM38 inhibits TNFα- and IL-1β-triggered NF-κB activation by mediating lysosome-dependent degradation of TAB2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Ming Hu,Qing Yang,Jing Zhang,Shi-Meng Liu,Yu Zhang,Heng Lin,Zhe-Fu Huang,Yan-Yi Wang,Xiao-Dong Zhang,Bo Zhong,Hong-Bing Shu
View all publications
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