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AB247981

Anti-TAB3 antibody [EPR5965] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAB3 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

MAP3K7IP3, TAB3, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, Mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NF-kappa-B-activating protein 1, TAK1-binding protein 3, TGF-beta-activated kinase 1-binding protein 3, TAB-3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)

This data was developed using ab124723, the same antibody clone in a different buffer formulation.ab124723, at 1/50 dilution, staining TAB3 in HepG2 cells by Immunofluorescence.

Western blot - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)
  • WB

Unknown

Western blot - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)

This data was developed using ab124723, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TAB3 antibody [EPR5965] (<a href='/en-us/products/primary-antibodies/tab3-antibody-epr5965-ab124723'>ab124723</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

OI-RD Scanning - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TAB3 antibody [EPR5965] - BSA and Azide free (AB247981)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5965

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247981 is the carrier-free version of ab124723.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAB3 also known as TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 is an essential protein that modulates important cellular processes. It has a molecular mass of approximately 90 kDa. The protein functions mechanically to activate downstream signaling pathways acting as an adapter molecule. Expressed ubiquitously TAB3 has more pronounced expression in tissues such as the liver suggesting its potential role in organ-specific functions.
Biological function summary

TAB3 influences multiple cellular signaling mechanisms contributing to the regulation of inflammatory responses and cell survival. It forms a part of important signaling complexes specifically the TAK1 complex which includes TAB1 and TAB2. Together this complex modulates pathways that are important for immune response and stress signaling in cells impacting the cell's ability to respond effectively to external signals.

Pathways

Research shows TAB3's involvement in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. As part of these pathways it interacts closely with other proteins including TAK1 orchestrating a coordinated cellular response to inflammatory and stress stimuli. The activity of these pathways is essential for maintaining proper cell signaling and gene expression under various physiological and pathological conditions.

Studies connect TAB3 to inflammatory and autoimmune diseases such as rheumatoid arthritis and inflammatory bowel disease. Its role alongside TAK1 and NF-κB in these conditions highlights its significance in managing inflammatory responses. Understanding TAB3’s interactions and regulation could offer insights into therapeutic targets for managing these autoimmune and inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (PubMed : 14633987, PubMed : 14766965, PubMed : 15327770, PubMed : 22158122, PubMed : 36593296). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2 : this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed : 15327770, PubMed : 18079694, PubMed : 22158122).. Isoform 2. May be an oncogenic factor.
See full target information TAB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao
View all publications
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Product promise

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