Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.
Images
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405183.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | IHC-P | mIHC | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Tested | Not recommended |
Dot
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/20000 | Notes - |
Species Rat | Dilution info 1/500 - 1/20000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Alternative names
Protachykinin-1, PPT, NKNA, NKA, TAC1, TAC2
Recommended products
Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.
Alternative names
Protachykinin-1, PPT, NKNA, NKA, TAC1, TAC2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR26567-18-1-3
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAC1 also known as Tachykinin Precursor 1 or Substance P is a gene that encodes a peptide which functions mechanically in neuropeptide signaling. TAC1 polypeptide has a mass of approximately 15 kDa. It is highly expressed in cells of the nervous system including the central and peripheral systems. The peptide derived from TAC1 participates in neurotransmission and modulates inflammatory responses.
- Biological function summary
TAC1 influences multiple processes primarily through interaction with the neurokinin receptors. The peptide is not part of a larger protein complex but it exerts its effects by binding to the tachykinin receptor NK1 which plays a role in regulating pain responses mood anxiety and the emetic reflex. TAC1-derived peptides are involved in promoting vasodilation and bronchoconstriction impacting cardiovascular and respiratory systems.
- Pathways
TAC1 acts in the tachykinin signaling pathway and affects various physiological processes. It is critical for the neurokinin pathway impacting the transmission of stress signals. TAC1 interacts closely with proteins like neurokinin-1 receptor (NK1R) and is involved in downstream effects such as calcium signaling pathways. These pathways highlight TAC1's role in communicating pain and stress stimuli across neurons.
- Associated diseases and disorders
TAC1 relates strongly to chronic pain and inflammatory diseases. Abnormal TAC1 expression has been linked to fibromyalgia and inflammatory bowel disease. In these conditions TAC1 peptides engage neuroinflammatory pathways often in association with other neuropeptides like CGRP (calcitonin gene-related peptide) leading to elevated inflammatory states. Understanding TAC1 interactions helps in strategizing therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405183.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405185.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of mouse spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405186.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of rat spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-ab307138--immunohistochemistry-img405184.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on mouse liver.
The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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