AB307138

Anti-TAC1 antibody [EPR26567-18-1-3]

BOND RX™ Validated
RabMAb
20ul selling size
Recombinant
Advanced Validation
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Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.

View Alternative Names

NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT

8 Images

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of mouse spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of rat spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue staining Mu Opioid Receptor with ab323645 at a 1 : 2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1 : 500 (0.966 ug/ml) dilution and ab316105 anti-GPCR GPR17 used at a 1 : 500 (0.509ug/ml) dilution.

Panel A : merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on rat spinal cord.
Panel B : anti-OPRM1 showed positive staining in rat spinal cord.
Panel C : anti-TAC1 showed positive staining in rat spinal cord.
Panel D : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323645, ab307138 and ab316105 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue staining Mu Opioid Receptor with ab323645 at a 1 : 2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1 : 500 (0.966 ug/ml) dilution and ab316105 anti-GPCR GPR17 used at a 1 : 500 (0.509ug/ml) dilution.

Panel A : merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on mouse spinal cord.
Panel B : anti-OPRM1 showed positive staining in mouse spinal cord.
Panel C : anti-TAC1 showed positive staining in mouse spinal cord.
Panel D : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in rat spinal cord.
Panel D : anti-ARMET/ARP staining neurons in rat spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Supplier Data

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse liver. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.
Panel D : anti-ARMET/ARP staining neurons in mouse spinal cord.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26567-18-1-3

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500 - 1/20000", "mIHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500 - 1/20000", "mIHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAC1 also known as Tachykinin Precursor 1 or Substance P is a gene that encodes a peptide which functions mechanically in neuropeptide signaling. TAC1 polypeptide has a mass of approximately 15 kDa. It is highly expressed in cells of the nervous system including the central and peripheral systems. The peptide derived from TAC1 participates in neurotransmission and modulates inflammatory responses.

Biological function summary

TAC1 influences multiple processes primarily through interaction with the neurokinin receptors. The peptide is not part of a larger protein complex but it exerts its effects by binding to the tachykinin receptor NK1 which plays a role in regulating pain responses mood anxiety and the emetic reflex. TAC1-derived peptides are involved in promoting vasodilation and bronchoconstriction impacting cardiovascular and respiratory systems.

Pathways

TAC1 acts in the tachykinin signaling pathway and affects various physiological processes. It is critical for the neurokinin pathway impacting the transmission of stress signals. TAC1 interacts closely with proteins like neurokinin-1 receptor (NK1R) and is involved in downstream effects such as calcium signaling pathways. These pathways highlight TAC1's role in communicating pain and stress stimuli across neurons.

TAC1 relates strongly to chronic pain and inflammatory diseases. Abnormal TAC1 expression has been linked to fibromyalgia and inflammatory bowel disease. In these conditions TAC1 peptides engage neuroinflammatory pathways often in association with other neuropeptides like CGRP (calcitonin gene-related peptide) leading to elevated inflammatory states. Understanding TAC1 interactions helps in strategizing therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.. Substance P. Is a ligand for TACR1, and triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding to TACR1 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity). Is also a TACR3 agonist with low receptor affinity (PubMed : 37391393). Basic secretagogue neuropeptide released from the terminals of specific sensory nerves (PubMed : 30686732). Acts as a ligand for MRGPRX2 receptor in mast cells, initiating a signaling that mediates neurogenic inflammation and pain (PubMed : 30686732, PubMed : 34789875). Neurogenic inflammation includes mast cell activation, recruitment of immune cells and release of inflammatory mediators, such as cytokines and chemokines (By similarity). The inflammatory response can then activate or sensitize nociceptors, promoting pain (By similarity).. Neurokinin A. Is a ligand for TACR2, and triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol hydrolysis by phospholipase C (PubMed : 35882833). Binding to TACR2 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP).

See full target information TAC1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAC1 antibody [EPR26567-18-1-3]

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise