Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)

Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	ICC/IF	IHC-P	mIHC	WB
Human	Not recommended	Not recommended	Tested	Expected	Not recommended
Mouse	Not recommended	Not recommended	Tested	Tested	Not recommended
Rat	Not recommended	Not recommended	Tested	Tested	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500 - 1/20000	Notes -
Species Rat	Dilution info 1/500 - 1/20000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Target data

See full target information TAC1

Function

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Alternative names

NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT

Recommended products

Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26567-18-1-3
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TAC1 also known as Tachykinin Precursor 1 or Substance P is a gene that encodes a peptide which functions mechanically in neuropeptide signaling. TAC1 polypeptide has a mass of approximately 15 kDa. It is highly expressed in cells of the nervous system including the central and peripheral systems. The peptide derived from TAC1 participates in neurotransmission and modulates inflammatory responses.

Biological function summary

TAC1 influences multiple processes primarily through interaction with the neurokinin receptors. The peptide is not part of a larger protein complex but it exerts its effects by binding to the tachykinin receptor NK1 which plays a role in regulating pain responses mood anxiety and the emetic reflex. TAC1-derived peptides are involved in promoting vasodilation and bronchoconstriction impacting cardiovascular and respiratory systems.

Pathways

TAC1 acts in the tachykinin signaling pathway and affects various physiological processes. It is critical for the neurokinin pathway impacting the transmission of stress signals. TAC1 interacts closely with proteins like neurokinin-1 receptor (NK1R) and is involved in downstream effects such as calcium signaling pathways. These pathways highlight TAC1's role in communicating pain and stress stimuli across neurons.

Associated diseases and disorders

TAC1 relates strongly to chronic pain and inflammatory diseases. Abnormal TAC1 expression has been linked to fibromyalgia and inflammatory bowel disease. In these conditions TAC1 peptides engage neuroinflammatory pathways often in association with other neuropeptides like CGRP (calcitonin gene-related peptide) leading to elevated inflammatory states. Understanding TAC1 interactions helps in strategizing therapeutic approaches for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue staining Mu Opioid Receptor with Anti-Mu Opioid Receptor antibody [EPR29112-126] ab323645 at a 1:2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1:500 (0.966 ug/ml) dilution and Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 anti-GPCR GPR17 used at a 1:500 (0.509ug/ml) dilution.
Panel A: merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on rat spinal cord.

Panel B: anti-OPRM1 showed positive staining in rat spinal cord.

Panel C: anti-TAC1 showed positive staining in rat spinal cord.

Panel D: anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Mu Opioid Receptor antibody [EPR29112-126] ab323645, ab307138 and Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue staining Mu Opioid Receptor with Anti-Mu Opioid Receptor antibody [EPR29112-126] ab323645 at a 1:2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1:500 (0.966 ug/ml) dilution and Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 anti-GPCR GPR17 used at a 1:500 (0.509ug/ml) dilution.
Panel A: merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on mouse spinal cord.

Panel B: anti-OPRM1 showed positive staining in mouse spinal cord.

Panel C: anti-TAC1 showed positive staining in mouse spinal cord.

Panel D: anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Mu Opioid Receptor antibody [EPR29112-126] ab323645, ab307138 and Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.

Panel B: anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.

Panel C: anti-TAC1 staining posterior horns (sensory) in rat spinal cord.

Panel D: anti-ARMET/ARP staining neurons in rat spinal cord.
The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26422-118] ab314307, ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.

Panel B: anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.

Panel C: anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.

Panel D: anti-ARMET/ARP staining neurons in mouse spinal cord.
The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26422-118] ab314307, ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of mouse spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of rat spinal cord. The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (ab307138)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TAC1 with ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: No staining on mouse liver.

The section was incubated with ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.