Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.
Images
![Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--multiplex-immunohistochemistry-img433039.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)")
![Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--multiplex-immunohistochemistry-img433038.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405183.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)")
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (AB307139)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | IHC-P | mIHC | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Tested | Not recommended |
Dot
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Alternative names
NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT
Recommended products
Rabbit Recombinant Monoclonal Protachykinin-1 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26567-18-1-3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAC1 also known as Tachykinin Precursor 1 or Substance P is a gene that encodes a peptide which functions mechanically in neuropeptide signaling. TAC1 polypeptide has a mass of approximately 15 kDa. It is highly expressed in cells of the nervous system including the central and peripheral systems. The peptide derived from TAC1 participates in neurotransmission and modulates inflammatory responses.
- Biological function summary
TAC1 influences multiple processes primarily through interaction with the neurokinin receptors. The peptide is not part of a larger protein complex but it exerts its effects by binding to the tachykinin receptor NK1 which plays a role in regulating pain responses mood anxiety and the emetic reflex. TAC1-derived peptides are involved in promoting vasodilation and bronchoconstriction impacting cardiovascular and respiratory systems.
- Pathways
TAC1 acts in the tachykinin signaling pathway and affects various physiological processes. It is critical for the neurokinin pathway impacting the transmission of stress signals. TAC1 interacts closely with proteins like neurokinin-1 receptor (NK1R) and is involved in downstream effects such as calcium signaling pathways. These pathways highlight TAC1's role in communicating pain and stress stimuli across neurons.
- Associated diseases and disorders
TAC1 relates strongly to chronic pain and inflammatory diseases. Abnormal TAC1 expression has been linked to fibromyalgia and inflammatory bowel disease. In these conditions TAC1 peptides engage neuroinflammatory pathways often in association with other neuropeptides like CGRP (calcitonin gene-related peptide) leading to elevated inflammatory states. Understanding TAC1 interactions helps in strategizing therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--multiplex-immunohistochemistry-img433039.jpg "Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (B), TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.
Panel B: anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Panel C: anti-TAC1 staining posterior horns (sensory) in rat spinal cord.
Panel D: anti-ARMET/ARP staining neurons in rat spinal cord.The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26422-118] ab314307, Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
![Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--multiplex-immunohistochemistry-img433038.jpg "Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Multiplex immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/2000 dilution (B), TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.
Panel B: anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C: anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.
Panel D: anti-ARMET/ARP staining neurons in mouse spinal cord.The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26422-118] ab314307, Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405183.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spinal cord. The section was incubated with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405185.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of mouse spinal cord. The section was incubated with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405186.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining predominanlty on dorsal horns of rat spinal cord. The section was incubated with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405184.jpg "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)
This data was developed using Anti-TAC1 antibody [EPR26567-18-1-3] ab307138, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 (0.966 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on mouse liver.
The section was incubated with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139), expandable thumbnail](https://content.abcam.com/products/images/tac1-antibody-epr26567-18-1-3-bsa-and-azide-free-ab307139--immunohistochemistry-img405184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] - BSA and Azide free (ab307139)")
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