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AB245455

Anti-TACC3 antibody

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(1 Publication)

Rabbit Polyclonal TACC3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TACC3 aa 400-500.

View Alternative Names

ERIC1, TACC3, Transforming acidic coiled-coil-containing protein 3, ERIC-1

2 Images
Immunoprecipitation - Anti-TACC3 antibody (AB245455)
  • IP

Supplier Data

Immunoprecipitation - Anti-TACC3 antibody (AB245455)

TACC3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245455 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245455 at 0.4 μg/ml.

Lane 1 : ab245455 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TACC3 antibody (ab245455)

Predicted band size: 90 kDa

false

Western blot - Anti-TACC3 antibody (AB245455)
  • WB

Ap

Western blot - Anti-TACC3 antibody (AB245455)

All lanes:

Western blot - Anti-TACC3 antibody (ab245455) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 90 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TACC3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6A5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245455 was affinity purified using an epitope specific to TACC3 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TACC3 also known as Transforming Acidic Coiled-Coil Containing Protein 3 is a microtubule-associated protein with a molecular weight of approximately 90 kDa. It plays an important role in stabilizing microtubules and is involved in mitotic spindle assembly. TACC3 is expressed in various tissues with high levels seen in proliferating cells such as embryonic cells and those in the testis. This expression pattern suggests it has important functions during cell division and development.
Biological function summary

TACC3 is part of a protein complex that contributes to mitosis. It collaborates with the Aurora A kinase and ch-TOG protein to ensure proper microtubule dynamics and spindle formation. This complex stabilizes kinetochore microtubules by connecting them to the centrosomes allowing accurate chromosome segregation during cell division. TACC3's actions are fundamental to cell cycle regulation and maintaining genomic stability.

Pathways

The role of TACC3 is linked to the cell cycle regulation and mitosis pathway. It interacts closely with the Aurora A kinase pathway playing a role in regulating centrosome maturation and spindle assembly. In these pathways proteins like TPX2 and PLK1 are also involved working alongside TACC3 to maintain the fidelity of cell division and ensure equal distribution of genetic material to daughter cells.

TACC3 is significantly implicated in various cancers. Abnormal expression levels of TACC3 have been associated with the development and progression of glioblastoma and breast cancer. It often exhibits partnerships with FGFR3 leading to oncogenic gene fusions that drive cancer cell growth and survival. Understanding TACC3 and its interactions remains important for developing targeted therapies in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed : 21297582, PubMed : 23532825). May be involved in the control of cell growth and differentiation. May contribute to cancer (PubMed : 14767476).
See full target information TACC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of bone oncology 33:100412 PubMed35198364

2022

MiR-451a promotes cell growth, migration and EMT in osteosarcoma by regulating YTHDC1-mediated m6A methylation to activate the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Cao,Shanshan Ge,Mengchun Li
View all publications
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