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Rabbit Recombinant Monoclonal TACI antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-TACI antibody [EPR23045-153] (AB239370), expandable thumbnail
  • Western blot - Anti-TACI antibody [EPR23045-153] (AB239370), expandable thumbnail
  • Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (AB239370), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.

Alternative names

CD267, TACI, TNFRSF13B, Tumor necrosis factor receptor superfamily member 13B, Transmembrane activator and CAML interactor

Recommended products

Rabbit Recombinant Monoclonal TACI antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23045-153
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Transmembrane Activator and CAML Interactor known as TACI also goes by the names TNFRSF13B and CD267. TACI is a receptor with a molecular weight of approximately 33 kDa. It is expressed on B cells a type of lymphocyte and to some extent on activated T cells. This receptor plays a part in the immune system helping manage the production and function of certain antibodies.

Biological function summary

TACI involves the regulation of B cell function and survival. It binds to two ligands APRIL and BAFF and TACI is not part of any larger complex. Upon ligand binding TACI can promote antibody class-switching which adjusts the type of antibody that B cells produce. This action supports the immune response by allowing the body to adapt to different pathogens effectively.

Pathways

Immune responses involve TACI in the BAFF-APRIL signaling pathway. The interplay between TACI and its ligands influences the NF-kB signaling cascade leading to B cell proliferation and survival. TACI interacts with other proteins such as BCMA and BAFF-R which are important in regulating B cell homeostasis and function ensuring balanced and effective immune responses.

Associated diseases and disorders

Aberrant TACI function contributes to conditions like Common Variable Immunodeficiency (CVID) and Systemic Lupus Erythematosus (SLE). In CVID mutations in the TACI gene may lead to impaired antibody production resulting in frequent infections. In the context of SLE TACI's interactions with BAFF and APRIL can contribute to the overactivation of B cells causing autoimmune characteristics of the disease. TACI's role in these conditions makes it a focus for studying therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-TACI antibody [EPR23045-153] (ab239370), expandable thumbnail

    Flow Cytometry - Anti-TACI antibody [EPR23045-153] (ab239370)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling TACI with ab239370 at 1/400 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab239370 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor 647.

    Gated on viable cells.

  • Western blot - Anti-TACI antibody [EPR23045-153] (ab239370), expandable thumbnail

    Western blot - Anti-TACI antibody [EPR23045-153] (ab239370)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 136 seconds.

    Negative control: U937 (PMID: 11104810).

    All lanes: Western blot - Anti-TACI antibody [EPR23045-153] (ab239370) at 1/1000 dilution

    Lane 1: IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte), whole cell at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

  • Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (ab239370), expandable thumbnail

    Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (ab239370)

    TACI was immunoprecipitated from 0.35 mg Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg with ab239370 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239370 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10μg

    Lane 2: ab239370 IP in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab239370 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (ab239370)

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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