AB181984

Anti-TADA3L antibody [EPR13864]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TADA3L antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ADA3, TADA3L, TADA3, Transcriptional adapter 3, ADA3 homolog, STAF54, Transcriptional adapter 3-like, hADA3, ADA3-like protein

2 Images

Flow Cytometry (Intracellular) - Anti-TADA3L antibody [EPR13864] (AB181984)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TADA3L antibody [EPR13864] (AB181984)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TADA3L with ab181984 at 1/90 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-TADA3L antibody [EPR13864] (AB181984)

All lanes:

Western blot - Anti-TADA3L antibody [EPR13864] (ab181984) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13864

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TADA3L also known as Transcriptional Adaptor 3-Like is part of the larger transcriptional coactivator complexes. The protein has a mass of approximately 55 kDa and is expressed in various tissues including liver and kidney. Mechanically TADA3L acts as a transcriptional adaptor interacting with DNA-binding transcription factors and assisting the recruitment of additional coactivators necessary for gene transcription.

Biological function summary

The protein assists in regulating gene expression by serving as a component of the human Ada-Two-A containing (hATAC) complex. The hATAC complex which includes TADA3L plays a role in histone acetylation allowing chromatin to be more accessible for transcriptional machinery. TADA3L's involvement in this complex underlines its role in modulating transcription and influencing cell cycle and proliferation processes.

Pathways

The TADA3L protein integrates into networks influencing transcriptional regulation and chromatin remodeling pathways. It associates with GCN5 and PCAF which are critical for histone acetyl transferase (HAT) activity within these pathways. Through its interaction with these proteins TADA3L contributes to the control of gene expression and is an important part of maintaining homeostasis in cellular function.

Alterations in TADA3L function or expression link to cancer and developmental disorders. Mutations or deregulation of this protein can disrupt normal transcriptional regulation leading to oncogenesis. In cancer pathways TADA3L shows connections with proteins like p53 a widely studied tumor suppressor where its impairment could affect apoptotic and cell cycle arrest pathways contributing to tumor development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.

See full target information TADA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 70:240-250 PubMed37005955

2023

Transcriptional adaptor 3 influences the proliferative and invasive phenotypes of non-small cell lung cancer cells via regulating EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Qin Xu,Shu-Wen Zhang,Rui Zhang,Jing-Jing Chen,Zai-Xin Yuan,Jian Feng,Jian-An Huang

Pathology, research and practice 215:152408 PubMed30954347

2019

Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwen Zhang,Liqin Xu,Zhiyuan Tang,Haiying Wang,Jun Gu,Baier Sun,Weishuai Zhang,Donghua Niu,Jian Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TADA3L antibody [EPR13864]

Flow Cytometry (Intracellular) - Anti-TADA3L antibody [EPR13864] (AB181984)

Western blot - Anti-TADA3L antibody [EPR13864] (AB181984)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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