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AB188427

Anti-TAF1 antibody

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(3 Publications)

Rabbit Polyclonal TAF1 antibody. Suitable for WB, ICC/IF and reacts with Human, Syrian hamster samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TAF1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BA2R, CCG1, CCGS, TAF2A, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (AB188427)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (AB188427)

Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.

Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (AB188427)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (AB188427)

IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.

Panel a) : Stained with ab188427 without immunizing peptide.
Panel b) : Stained with ab188427 with immunizing peptide.
Panel c) : Panel a) co-stained with Hoechst 33258.
Panel d) : Panel b) co-stained with Hoechst 33258.
Panel e) : Phase-contrast image of panels a) and c).
Panel f) : Phase-contrast image of panels b) and d).

Western blot - Anti-TAF1 antibody (AB188427)
  • WB

Supplier Data

Western blot - Anti-TAF1 antibody (AB188427)

All lanes:

Western blot - Anti-TAF1 antibody (ab188427) at 1/1000 dilution

All lanes:

HeLa whole cell extract at 10 µg

Predicted band size: 213 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Syrian hamster

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TAF1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P21675

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/5000", "ICCIF-species-notes": "<p></p>" }, "Syrian hamster": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF1 also known as TATA-box binding protein associated factor 1 has a mechanical role as a transcription factor in the initiation process of transcription. This protein forms a part of the larger transcription factor IID (TFIID) complex weighing approximately 200 kDa. TAF1 expresses broadly in tissues where it plays an important role in mediating transcriptional regulation. It is a general transcription factor that serves as a molecular scaffold essential for recruiting the necessary machinery to specific promotor regions of DNA for transcription activation.
Biological function summary

TAF1 interacts extensively with other TFIID components to control the formation of the pre-initiation complex on core promoters. This protein is integral to the regulation of gene expression by modulating transcriptional activity in cells. TAF1 acts as a histone acetyltransferase and a kinase modifying other proteins' functions and structural capabilities in the transcription process. Its enzymatic activities make it essential for the phosphorylation and acetylation of histones directly impacting the accessibility of chromatin for transcription.

Pathways

TAF1 functions prominently in the RNA polymerase II transcription initiation pathway. Its interactions with TATA-binding protein and other TAFs facilitate the robust expression of genes essential for various cellular functions. TAF1 establishes connections with proteins like TBP acting within the canonical pathway that guides precise transcription initiation. Additionally TAF1 partakes in the p53 signaling pathway where it interacts with several p53 regulators influencing cell cycle regulation and apoptosis.

TAF1 mutations are linked to X-linked dystonia-parkinsonism and certain neurodegenerative disorders. Aberrations in TAF1 function impact transcriptional regulation leading to dysregulation in neuronal gene expression. Furthermore TAF1 associations with Huntington's disease have been documented highlighting its role in neuronal survival and stress response. In these diseases altered pathways implicate TAF1 with other proteins such as Huntingtin underlining its importance in maintaining neuronal health and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed : 25412659, PubMed : 27007846, PubMed : 33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed : 33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed : 25412659, PubMed : 8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed : 25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed : 25412659). Possesses DNA-binding activity (PubMed : 25412659). Essential for progression of the G1 phase of the cell cycle (PubMed : 11278496, PubMed : 15053879, PubMed : 2038334, PubMed : 8450888, PubMed : 8625415, PubMed : 9660973, PubMed : 9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed : 15870300).
See full target information TAF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 30:1141-1152 PubMed37386215

2023

Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Bernardini,Pooja Mukherjee,Elisabeth Scheer,Ivanka Kamenova,Simona Antonova,Paulina Karen Mendoza Sanchez,Gizem Yayli,Bastien Morlet,H T Marc Timmers,László Tora

Journal of extracellular vesicles 11:e12185 PubMed35029057

2022

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yu,Ying Bai,Bing Han,Minzi Ju,Tianci Tang,Ling Shen,Mingyue Li,Li Yang,Zhao Zhang,Guoku Hu,Jie Chao,Yuan Zhang,Honghong Yao

Molecular and cellular biology 11:3317-25 PubMed2038334

1991

The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

T Sekiguchi,Y Nohiro,Y Nakamura,N Hisamoto,T Nishimoto
View all publications
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Product promise

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