AB263967

Anti-TAF10 antibody

Name: Anti-TAF10 antibody (ab263967) | Abcam
Brand: Abcam Limited
SKU: AB263967
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal TAF10 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TAF10.

View Alternative Names

TAF2A, TAF2H, TAFII30, TAF10, Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30 kDa subunit, TAF(II)30, TAFII-30

1 Images

Supplier Data

Western blot - Anti-TAF10 antibody (AB263967)

Primary incubation overnight at 4°C.

Secondary incubation for 45 minutes at RT.

All lanes:

Western blot - Anti-TAF10 antibody (ab263967) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 2:

HeLa nuclear extract at 35 µg

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract at 35 µg

Lane 4:

PANC-1 (human pancreatic epithelial cancinoma cell line) whole cell lysate at 35 µg

Lane 5:

Mouse brain whole cell lysate at 35 µg

Secondary

All lanes:

Rabbit secondary HRP at 1/70000 dilution

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human TAF10. The exact immunogen used to generate this antibody is proprietary information.

Q12962

Specificity

A BLAST analysis was used to suggest cross reactivity with TAF10 protein from human and mouse (100% homology). Reactivity against homologues from other sources is not known.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity-purified antibody is directed against human TAF10 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF10 also known as TATA-box binding protein associated factor 10 is a component of the transcription machinery. It has a molecular weight of approximately 21 kDa. This protein is involved in assembling the general transcription factors necessary for the transcription of class II genes. TAF10 is broadly expressed in many tissues particularly in cells that undergo frequent transcriptional activity such as those in the liver and kidney.

Biological function summary

TAF10 acts as an integral part of the transcription factor IID (TFIID) complex which is essential for RNA polymerase II-driven transcription initiation. TAF10 contributes to the recognition and binding of promoter DNA sequences facilitating transcription initiation. It also serves a regulatory role in mediating interactions between the TFIID complex and other transcription factors enhancing transcriptional control and efficiency.

Pathways

TAF10 plays an important role in the regulation of gene expression and RNA polymerase II transcription pathways. It interacts with various components of the transcription initiation complex to initiate and regulate transcription. TAF10 associates with other TAFs and TBP (TATA-binding protein) in the TFIID complex together forming a scaffold for the correct assembly of RNA polymerase II.

TAF10 has been associated with neurodegenerative disorders and certain cancers. Mutations or dysregulation of TAF10 can lead to disruptions in gene expression contributing to the pathogenesis of these conditions. In the case of cancer TAF10 intersects with tumor suppressor proteins such as p53 influencing cell cycle regulation and tumor growth. These interactions highlight TAF10's importance in maintaining cellular homeostasis and its potential role in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF10 is also component of the PCAF histone acetylase complex, the TATA-binding protein-free TAF complex (TFTC) and the STAGA transcription coactivator-HAT complex (PubMed : 10373431, PubMed : 11564863, PubMed : 12601814, PubMed : 18206972, PubMed : 9885574). May regulate cyclin E expression (By similarity).

See full target information TAF10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Human genetics 143:683-694 PubMed38592547

2024

Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kopp,Leonard A Koch,Hristiana Lyubenova,Oliver Küchler,Manuel Holtgrewe,Andranik Ivanov,Christele Dubourg,Erika Launay,Sebastian Brachs,Stefan Mundlos,Nadja Ehmke,Dominik Seelow,Mélanie Fradin,Uwe Kornak,Björn Fischer-Zirnsak

Journal of oncology 2022:8890434 PubMed35664563

2022

Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4CD25FOXP3 Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Renyong Wang,Ruixue Li,Tiehan Li,Lei Zhu,Zongze Qi,Xiaokui Yang,Huan Wang,Baoquan Cao,Hong Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAF10 antibody

Western blot - Anti-TAF10 antibody (AB263967)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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