AB133760

Anti-TAF15 antibody [EPR9196(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TAF15 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RBP56, TAF2N, TAF15, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, RNA-binding protein 56, TAF(II)68, TAFII68

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunofluorescent staining of TAF15 in 293T cells stained with ab133760 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunohistochemical analysis using ab133760 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunohistochemical analysis using ab133760 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunohistochemical analysis using ab133760 showing positive staining in Lung adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunohistochemical analysis of TAF15 in paraffin embedded Human kidney tissue stained with ab133760 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

Immunohistochemical analysis using ab133760 showing positive staining in Urinary bladder transitional carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

WB

Unknown

Western blot - Anti-TAF15 antibody [EPR9196(B)] (AB133760)

All lanes:

Western blot - Anti-TAF15 antibody [EPR9196(B)] (ab133760) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 62 kDa

false

Carrier free

Anti-TAF15 antibody [EPR9196(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9196(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Perform antigen retrieval before commencing with IHC staining protocol.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF15 also known as TATA-binding protein-associated factor 15 is a RNA polymerase II-associated factor with approximate mass of 56 kDa. It belongs to the FET family of proteins which include FUS and EWS. This protein expresses widely in various tissues including the brain and immune cells. Mechanically TAF15 participates in transcription by mediating interactions between transcription factors and the RNA polymerase II complex.

Biological function summary

The protein helps regulate transcription initiation and elongation. TAF15 operates as a part of the transcription pre-initiation complex working closely with other transcription-associated factors to control gene expression. Its role extends beyond the nucleus as it may participate in RNA processing and transport due to its ability to bind RNA.

Pathways

TAF15 plays a role in the regulation of transcription from RNA polymerase II promoters. It interacts with numerous other proteins such as TFIID and RNA polymerase II subunits facilitating transcriptional regulation. TAF15 also associates with pathways related to stress response and cellular growth emphasizing its role in maintaining cellular integrity and function.

TAF15 links to certain forms of cancer and neurodegenerative diseases. Its misregulation or mutation associates with some sarcomas indicating a role in tumorigenesis possibly through interactions with oncogenic proteins like EWS. In neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) TAF15 might be implicated potentially through pathways shared with proteins like TDP-43 that are involved in RNA metabolism dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).

See full target information TAF15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental pathology 7:64-79 PubMed24427327

2014

The onset of human ectopic pregnancy demonstrates a differential expression of miRNAs and their cognate targets in the Fallopian tube.

Applications

IHC

Species

Unspecified reactive species

Yi Feng,Shien Zou,Birgitta Weijdegård,Jie Chen,Qing Cong,Julia Fernandez-Rodriguez,Lei Wang,Håkan Billig,Ruijin Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com