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AB250064

Anti-TAF1C antibody [EPR11298] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAF1C antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TATA box-binding protein-associated factor RNA polymerase I subunit C, RNA polymerase I-specific TBP-associated factor 110 kDa, TATA box-binding protein-associated factor 1C, Transcription initiation factor SL1/TIF-IB subunit C, TAFI110, TBP-associated factor 1C, TAF1C

2 Images
Immunoprecipitation - Anti-TAF1C antibody [EPR11298] - BSA and Azide free (AB250064)
  • IP

Supplier Data

Immunoprecipitation - Anti-TAF1C antibody [EPR11298] - BSA and Azide free (AB250064)

This data was developed using ab178690, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab178690.

All lanes:

Immunoprecipitation - Anti-TAF1C antibody [EPR11298] (<a href='/en-us/products/primary-antibodies/taf1c-antibody-epr11298-ab178690'>ab178690</a>)

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Western blot - Anti-TAF1C antibody [EPR11298] - BSA and Azide free (AB250064)
  • WB

Supplier Data

Western blot - Anti-TAF1C antibody [EPR11298] - BSA and Azide free (AB250064)

This data was developed using ab178690, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TAF1C antibody [EPR11298] (<a href='/en-us/products/primary-antibodies/taf1c-antibody-epr11298-ab178690'>ab178690</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11298

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab250064 is the carrier-free version of ab178690.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF1C also known as Transcription Initiation Factor TFIID Subunit 1C is a component of the transcription factor complex that plays an important role in the initiation of transcription by RNA polymerase I. It has a molecular mass of approximately 64 kDa. TAF1C is mainly expressed in the nucleolus of eukaryotic cells where it aids in forming the transcription preinitiation complex. The protein interacts specifically with other subunits to enable the RNA polymerase I to initiate the transcription of ribosomal RNA genes.
Biological function summary

TAF1C is essential for assembling the RNA polymerase I complex often referred to as the SL1 complex. This complex includes other components like TBP and several TAFs which together promote the transcription of rRNA genes critical for ribosome biogenesis. By participating in the formation of this complex TAF1C impacts cellular growth by influencing ribosome production. The functional activity of TAF1C thereby strongly ties to cellular metabolism given its position in rRNA transcription machinery.

Pathways

TAF1C is involved in the rRNA synthesis pathway a pathway important for ribosome production. This pathway interfaces with the cell growth and division pathways with proteins like TBP and other transcription factors forming significant parts of these linked networks. TAF1C contributes to maintaining cellular homeostasis by its functions in these pathways impacting how cells respond and adapt to metabolic demands and growth signals.

TAF1C has connections to diseases like cancer due to its involvement in ribosome biogenesis. Altered expression of TAF1C affects nucleolar activity which is often observed in tumor cells. Moreover any disruption in its function can lead to dysregulation of rRNA synthesis linking it indirectly to p53 a protein involved in cell cycle regulation and apoptosis. Aberrant regulation or mutations in TAF1C-related pathways may fuel disease progression and are subjects of current research interest in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.
See full target information TATA box-binding protein-associated factor RNA polymerase I subunit C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 3: PubMed25206958

2014

Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.

Applications

Unspecified application

Species

Unspecified reactive species

Marijke I Zonneveld,Alain R Brisson,Martijn J C van Herwijnen,Sisareuth Tan,Chris H A van de Lest,Frank A Redegeld,Johan Garssen,Marca H M Wauben,Esther N M Nolte-'t Hoen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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