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AB189428

Anti-TAF4B antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal TAF4B antibody. C-terminal. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TAF4B aa 650-700 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TAF2C2, TAFII105, TAF4B, Transcription initiation factor TFIID subunit 4B, Transcription initiation factor TFIID 105 kDa subunit, TAF(II)105, TAFII-105

3 Images
Flow Cytometry (Intracellular) - Anti-TAF4B antibody - C-terminal (AB189428)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TAF4B antibody - C-terminal (AB189428)

Intracellular flow cytometric analysis of K562 cells labeling TAF4B with ab189428 at 1/10 dilution (right histogram) compared to negative control cells (left histogram) using FITC-conjugated goat-anti-rabbit secondary antibodies.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF4B antibody - C-terminal (AB189428)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF4B antibody - C-terminal (AB189428)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling TAF4B with ab189428 at 5 μg/ml.

Western blot - Anti-TAF4B antibody - C-terminal (AB189428)
  • WB

Supplier Data

Western blot - Anti-TAF4B antibody - C-terminal (AB189428)

All lanes:

Western blot - Anti-TAF4B antibody - C-terminal (ab189428) at 1/1000 dilution

All lanes:

K562 cell lysate at 35 µg

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human TAF4B aa 650-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q92750

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF4B also known as TBP-associated factor 4B is a protein important in transcription initiation. This protein is part of the TFIID complex and has a mass of approximately 85 kDa. It is expressed in various tissues including ovaries and testes. TAF4B facilitates interactions between TBP (TATA-binding protein) and other transcription factors promoting the assembly of pre-initiation complexes on gene promoters.
Biological function summary

TAF4B has significant roles in regulating gene expression. It forms part of the multi-subunit TFIID complex. Through this association TAF4B is involved in the transcription of genes required for cell cycle progression and development. Its expression levels and regulation affect cellular differentiation and growth making it essential for normal cellular functions especially in reproductive tissues.

Pathways

TAF4B participates in key biological processes such as the RNA polymerase II pre-initiation complex formation. It interacts with other TAFs (TBP-associated factors) and transcriptional activators that modulate gene expression in response to cellular signals. In particular the estrogen signaling pathway has a link with TAF4B influencing gene transcription in reproductive tissues. This protein works closely with other components of the TFIID complex to ensure accurate transcriptional regulation.

Alterations in TAF4B expression or function associate with conditions like infertility and ovarian dysfunction. In reproductive disorders its expression levels may impact genes imperative for gonadal development. TAF4B's interaction with estrogen receptors also suggests potential connections to disorders influenced by hormonal imbalances though more studies are needed to fully define these links and associated proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.
See full target information TAF4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 19:2793-2810 PubMed33064976

2020

Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of .

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Jing Hao,Cheng-Quan Yan,He-Feng Wang,Bin Meng,Sheng-Yong Cai
View all publications
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Product promise

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