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AB251684

Anti-TAF5 antibody

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(1 Publication)

Rabbit Polyclonal TAF5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TAF5 aa 300-450.

View Alternative Names

TAF2D, TAF5, Transcription initiation factor TFIID subunit 5, Transcription initiation factor TFIID 100 kDa subunit, TAF(II)100, TAFII-100, TAFII100

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAF5 antibody (AB251684)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAF5 antibody (AB251684)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TAF5 (green) using ab251684 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF5 antibody (AB251684)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF5 antibody (AB251684)

Paraffin-embedded human cerebral cortex tissue stained for TAF5 using ab251684 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-TAF5 antibody (AB251684)
  • WB

Supplier Data

Western blot - Anti-TAF5 antibody (AB251684)

All lanes:

Western blot - Anti-TAF5 antibody (ab251684) at 0.4 µg/mL

All lanes:

U937 MG (human histiocytic lymphoma cell line) cell lysate

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TAF5 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q15542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF5 also known as TAFII100 is a component of the transcription factor IID complex and is vital in the process of assembling the pre-initiation complex for gene transcription. This protein has a molecular mass of approximately 100 kDa. It is prominently expressed in a variety of tissues such as liver and heart indicating its broad functional significance in various cellular environments. TAF5 through its structural role helps stabilize the TFIID complex facilitating the binding of transcription machinery to DNA promoters.
Biological function summary

TAF5 forms part of the TFIID complex a cornerstone for gene transcription initiation. TFIID is one of the basal transcription factors essential for RNA polymerase II to carry out transcription of mRNA. The proper assembly and functioning of TFIID rely heavily on TAF5 as it interacts with other TAFs and the TBP (TATA-box Binding Protein) enabling accurate transcriptional regulation. This process impacts the expression of a large number of genes highlighting TAF5's broad biological utility and influence.

Pathways

TAF5 plays a major role in the transcription initiation pathway and is an active participant in the RNA polymerase II transcription machinery. It associates closely with TAF6 and TAF9 within the TFIID complex facilitating the recruitment of other proteins to initiate transcription. Through these interactions TAF5 impacts the cell cycle control and apoptosis pathways modulating cellular responses to developmental cues and environmental stresses. These pathways illustrate the importance of TAF5 in maintaining cellular homeostasis and responding to physiological signals.

TAF5 has associations with cancer and certain neurodegenerative diseases. Aberrant expression or mutations in the TAF5 gene may disrupt the transcriptional machinery leading to uncontrolled cellular proliferation or neuronal cell death. For example in cancer TAF5's interaction with TIP60 a histone acetyltransferase modifies chromatin structure influencing tumorigenic processes. Meanwhile in neurodegenerative disorders impaired TAF5 functions might alter gene expression patterns important for neuronal survival linking TAF5 to the disease pathology and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473, PubMed : 8758937, PubMed : 8942982, PubMed : 9045704). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TAF5 is involved in two modules of TFIID, in TFIID-A together with TAF3 and TBP, and in TFIID-B with TAF8 (PubMed : 33795473). Involved in contacts between TFIID and TFIIF in the PIC (PubMed : 33795473).
See full target information TAF5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:28 PubMed40045194

2025

tRF-5028c disrupts trophoblast function in recurrent spontaneous abortion by inhibiting CRKL-mediated Rap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jialyu Huang,Jiawei Wang,Shuang Wang,Xiangpeng Xiong,Ruiyin Jiang,Chaoyi Xiong,Lu Wang,Lingling Huang,Yan Zhao,Zheng Fang,Xiaoyan Ai,Jiaying Lin
View all publications
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