AB172621

Anti-TAF5L antibody [EPR11445]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TAF5L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PAF65B, TAF5L, TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PAF65-beta

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TAF5L antibody [EPR11445] (AB172621)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAF5L antibody [EPR11445] (AB172621)

Immunofluorescent staining of HeLa cells labeling TAF5L with ab172621 at 1/100 dilution.

Supplier Data

Western blot - Anti-TAF5L antibody [EPR11445] (AB172621)

All lanes:

Western blot - Anti-TAF5L antibody [EPR11445] (ab172621) at 1/1000 dilution

Lane 1:

HT-29 lysate at 10 µg

Lane 2:

HT-1080 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Predicted band size: 66 kDa

false

Carrier free

Anti-TAF5L antibody [EPR11445] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11445

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF5L also known as Transcription initiation factor TFIID subunit 5-like is a protein that plays an important role in gene expression through its involvement in the transcription machinery. It weighs approximately 80 kDa and is composed of 787 amino acids. TAF5L is expressed in various tissues with higher levels in the spleen thymus and bone marrow. This expression pattern indicates its role in processes that require precise transcription regulation.

Biological function summary

TAF5L functions as a fundamental unit of the transcription factor IID (TFIID) complex. This complex comprises several other TAF proteins and the TATA-binding protein (TBP) orchestrating the assembly of the preinitiation complex (PIC) on promoters. By stabilizing interactions between these proteins TAF5L facilitates the precise transcription initiation of various genes. Its role in the TFIID complex makes it essential for the recruitment of RNA polymerase II to specific DNA regions.

Pathways

TAF5L actively participates in the RNA polymerase II transcription initiation pathway and the regulation of cellular response to DNA damage. Within these pathways its interaction with other TAF subunits and the TBP coordinates transcription activities. TAF5L specifically teams up with TAF6 to support the structural integrity of the TFIID complex ensuring effective transcription of genes in response to developmental and environmental signals.

TAF5L shows connections to certain cancers including colorectal and breast cancer. Alterations in TAF5L expression or function can lead to dysregulation of gene transcription contributing to tumorigenesis and cancer progression. The protein's association with the p53 signaling pathway responsible for maintaining genomic stability further highlights its importance in cancer. Additionally disruptions in TAF5L interactions with other TAF proteins or TBP may impact cell cycle control further linking it to cancer development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF6L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state (By similarity).

See full target information TAF5L

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:255 PubMed40448099

2025

C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shriya Joshi,Chakravarthy Garlapati,Thi Nguyen,Shaligram Sharma,Darshan Shimoga Chandrashekar,Shristi Bhattarai,Sooryanarayana Varambally,Uma Krishnamurti,Xiaoxian Li,Ritu Aneja

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAF5L antibody [EPR11445]

Immunocytochemistry/ Immunofluorescence - Anti-TAF5L antibody [EPR11445] (AB172621)

Western blot - Anti-TAF5L antibody [EPR11445] (AB172621)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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