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AB249714

Anti-TAF5L antibody [EPR11445] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAF5L antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PAF65B, TAF5L, TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PAF65-beta

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAF5L antibody [EPR11445] - BSA and Azide free (AB249714)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAF5L antibody [EPR11445] - BSA and Azide free (AB249714)

This data was developed using ab172621, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HeLa cells labeling TAF5L with ab172621 at 1/100 dilution.

Western blot - Anti-TAF5L antibody [EPR11445] - BSA and Azide free (AB249714)
  • WB

Supplier Data

Western blot - Anti-TAF5L antibody [EPR11445] - BSA and Azide free (AB249714)

This data was developed using ab172621, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TAF5L antibody [EPR11445] (<a href='/en-us/products/primary-antibodies/taf5l-antibody-epr11445-ab172621'>ab172621</a>) at 1/1000 dilution

Lane 1:

HT-29 lysate at 10 µg

Lane 2:

HT-1080 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11445

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249714 is the carrier-free version of ab172621.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF5L also known as Transcription initiation factor TFIID subunit 5-like is a protein that plays an important role in gene expression through its involvement in the transcription machinery. It weighs approximately 80 kDa and is composed of 787 amino acids. TAF5L is expressed in various tissues with higher levels in the spleen thymus and bone marrow. This expression pattern indicates its role in processes that require precise transcription regulation.
Biological function summary

TAF5L functions as a fundamental unit of the transcription factor IID (TFIID) complex. This complex comprises several other TAF proteins and the TATA-binding protein (TBP) orchestrating the assembly of the preinitiation complex (PIC) on promoters. By stabilizing interactions between these proteins TAF5L facilitates the precise transcription initiation of various genes. Its role in the TFIID complex makes it essential for the recruitment of RNA polymerase II to specific DNA regions.

Pathways

TAF5L actively participates in the RNA polymerase II transcription initiation pathway and the regulation of cellular response to DNA damage. Within these pathways its interaction with other TAF subunits and the TBP coordinates transcription activities. TAF5L specifically teams up with TAF6 to support the structural integrity of the TFIID complex ensuring effective transcription of genes in response to developmental and environmental signals.

TAF5L shows connections to certain cancers including colorectal and breast cancer. Alterations in TAF5L expression or function can lead to dysregulation of gene transcription contributing to tumorigenesis and cancer progression. The protein's association with the p53 signaling pathway responsible for maintaining genomic stability further highlights its importance in cancer. Additionally disruptions in TAF5L interactions with other TAF proteins or TBP may impact cell cycle control further linking it to cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF6L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state (By similarity).
See full target information TAF5L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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