AB51026

Anti-TAF6 antibody [585D4a]

Name: Anti-TAF6 antibody [585D4a] (ab51026) | Abcam
Brand: Abcam Limited
SKU: AB51026

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(2 Publications)

Mouse Monoclonal TAF6 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TAF6.

View Alternative Names

TAF2E, TAFII70, TAF6, Transcription initiation factor TFIID subunit 6, RNA polymerase II TBP-associated factor subunit E, Transcription initiation factor TFIID 70 kDa subunit, Transcription initiation factor TFIID 80 kDa subunit, TAF(II)70, TAFII-70, TAF(II)80, TAFII-80, TAFII80

3 Images

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Immunocytochemistry - Anti-TAF6 antibody [585D4a] (AB51026)

HeLa cells labelled with ab51026 at 1/5 dilution. Alexa Fluor 488 Goat anti-Mouse IgG was used as secondary antibody at 1/200 dilution.

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Immunoprecipitation - Anti-TAF6 antibody [585D4a] (AB51026)

ab51026 was used at 5µg/500µg of RIPA lysate of HeLa cells. Western blot was labelled with ab51026 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution and developed using the ECL technique.

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Immunoprecipitation - Anti-TAF6 antibody [585D4a] (ab51026)

Predicted band size: 73 kDa

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Western blot - Anti-TAF6 antibody [585D4a] (AB51026)

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Western blot - Anti-TAF6 antibody [585D4a] (ab51026) at 1/50 dilution

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HEK293 whole cell lysate at 25 µg

Secondary

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Mouse IgG antibody at 1/2500 dilution

Predicted band size: 73 kDa

Observed band size: 105 kDa,73 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

585D4a

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC, IP

applications

Immunogen

Recombinant Fragment Protein within Human TAF6. The exact immunogen used to generate this antibody is proprietary information.

P49848

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/5", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified using protein G chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF6 also known as TAFII70 is a component of the transcription factor IID (TFIID) complex with a mass of about 70 kDa. This protein plays an important role in the initiation of gene transcription by RNA polymerase II. It is expressed in various tissues throughout the body. TAF6 interacts with core promoter elements to facilitate the recruitment of other transcriptional machinery components.

Biological function summary

TAF6 functions as part of the larger TFIID complex which is essential for transcription initiation. The TFIID complex assembles at the core promoter and helps in the recognition and binding of sequence-specific DNA sites. TAF6 contributes to the structural integrity and functional specificity of the complex. These interactions are fundamental for maintaining proper gene expression patterns within cells.

Pathways

TAF6 is integral to transcription regulation pathways such as those initiating from the TATA box within promoters. It works closely with other TFIID components like TATA-binding protein (TBP) and TAF1. Through these interactions TAF6 ensures the correct positioning of RNA polymerase II for efficient transcription initiation. Another pathway involving TAF6 is the MAPK signaling pathway where TAF6 can influence transcriptional responses to external signals.

Alterations in TAF6 expression or function have been implicated in certain cancers such as colorectal cancer. TAF6 can also interact with p53 a well-known tumor suppressor protein linking it to cancer progression pathways. Mutations or dysregulation of TAF6 may affect p53-mediated stress responses contributing to tumor development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TAF6 homodimer connects TFIID modules, forming a rigid core (PubMed : 33795473).. Isoform 4. Transcriptional regulator which acts primarily as a positive regulator of transcription (PubMed : 20096117, PubMed : 29358700). Recruited to the promoters of a number of genes including GADD45A and CDKN1A/p21, leading to transcriptional up-regulation and subsequent induction of apoptosis (PubMed : 11583621). Also up-regulates expression of other genes including GCNA/ACRC, HES1 and IFFO1 (PubMed : 18628956). In contrast, down-regulates transcription of MDM2 (PubMed : 11583621). Acts as a transcriptional coactivator to enhance transcription of TP53/p53-responsive genes such as DUSP1 (PubMed : 20096117). Can also activate transcription and apoptosis independently of TP53 (PubMed : 18628956). Drives apoptosis via the intrinsic apoptotic pathway by up-regulating apoptosis effectors such as BCL2L11/BIM and PMAIP1/NOXA (PubMed : 29358700).

See full target information TAF6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 287:23035-45 PubMed22577136

2012

Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.

Applications

Species

Unspecified reactive species

Dotan Sela,Lu Chen,Skylar Martin-Brown,Michael P Washburn,Laurence Florens,Joan Weliky Conaway,Ronald C Conaway

The Journal of biological chemistry 284:32405-12 PubMed19805548

2009

Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID.

Applications

Species

Human

Hidehisa Takahashi,Skylar Martin-Brown,Michael P Washburn,Laurence Florens,Joan W Conaway,Ronald C Conaway

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TAF6 antibody [585D4a]

Immunocytochemistry - Anti-TAF6 antibody [585D4a] (AB51026)

Immunoprecipitation - Anti-TAF6 antibody [585D4a] (AB51026)

Western blot - Anti-TAF6 antibody [585D4a] (AB51026)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise