Anti-TAF7 antibody [2C5]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF7 antibody [2C5] (AB57494)

TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-TAF7 antibody [2C5] (AB57494)

Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This image was generated using the ascites version of the product.

• IHC-P

AbReview18698****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF7 antibody [2C5] (AB57494)

ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

This image was generated using the ascites version of the product.

This image was kindly supplied by Dr Karin Birkenkamp-Demtroeder by Abreview

• WB

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Western blot - Anti-TAF7 antibody [2C5] (AB57494)

TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-TAF7 antibody [2C5] (ab57494)

Predicted band size: 40 kDa

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• WB

AbReview18699****

Western blot - Anti-TAF7 antibody [2C5] (AB57494)

Lane 1 : All Blue Biorad.Gel run under denaturing conditions with a 4-12% gradient.Blocking step performed using 5% milk for one hour at room temperature.Primary antibody incubated for 16 hours.

This image was generated using the ascites version of the product.

Lane 2:

Western blot - Anti-TAF7 antibody [2C5] (ab57494) at 1/1250 dilution

Lane 3:

Western blot - Anti-TAF7 antibody [2C5] (ab57494) at 1/2500 dilution

Lane 4:

Western blot - Anti-TAF7 antibody [2C5] (ab57494) at 1/5000 dilution

Lane 5:

Western blot - Anti-TAF7 antibody [2C5] (ab57494) at 1/10000 dilution

All lanes:

whole cell lysate prepared from SW780 badder cancer cells at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG conjugated to HRP

Predicted band size: 40 kDa

Observed band size: 40 kDa,90 kDa

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Exposure time: 10min

Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview