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AB204894

Anti-TAF8/TBN antibody

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(4 Publications)

Rabbit Polyclonal TAF8/TBN antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TAF8 aa 50-200.

View Alternative Names

TAFII43, TBN, TAF8, Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, hTAFII43

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF8/TBN antibody (AB204894)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF8/TBN antibody (AB204894)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TAF8/TBN with ab204894 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TAF8/TBN antibody (AB204894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAF8/TBN antibody (AB204894)

Immunofluorescent analysis of U-251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling TAF8/TBN with ab204894 at 4 μg/ml (green).

Western blot - Anti-TAF8/TBN antibody (AB204894)
  • WB

Supplier Data

Western blot - Anti-TAF8/TBN antibody (AB204894)

All lanes:

Western blot - Anti-TAF8/TBN antibody (ab204894) at 1/100 dilution

Lane 1:

Negative Control

Lane 2:

HEK293 lysate

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TAF8 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q7Z7C8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF8 also known as TAF2G or TBN is a protein involved in transcription regulation. It has a molecular mass of approximately 38 kDa. TAF8 mainly expresses in the cell nucleus where it is an essential component of the transcription machinery. The protein plays a mechanical role in assembling and stabilizing the structure of the TFIID complex which is critical for initiating RNA polymerase II-dependent transcription.
Biological function summary

TAF8 associates with other TFIID components to influence gene expression. It is a part of the TFIID multi-protein complex that mediates the binding of the transcription factor IID (TFIID) to specific promoters. This complex interaction allows TAF8 to facilitate the transcription of various genes. It also interacts with transcription factors and other co-activators playing an essential role in regulating cellular transcription machinery.

Pathways

TAF8 functions within the transcription initiation pathway and directly impacts the transcription of protein-coding genes. It works alongside proteins like TBP and other TAFs within this pathway to form a backbone for the general transcription machinery. Its interactions help regulate the accessibility of DNA to RNA polymerase II and modulate the transcriptional activity of several important genes in the cell.

Defects in pathways involving TAF8 have links to certain developmental abnormalities and transcription-related disorders. For example mutations affecting TAF8 can contribute to diseases such as certain cancer types where precise control of transcription is disrupted. TAF8's connection with other TAF proteins within the TFIID complex accentuates its role in maintaining normal cellular function and preventing transcription-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TAF8 is involved in forming the TFIID-B module, together with TAF5 (PubMed : 33795473). Mediates both basal and activator-dependent transcription (PubMed : 14580349). Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts (PubMed : 14580349). Required for the integration of TAF10 in the TAF complex (PubMed : 14580349). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity).
See full target information TAF8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2083-2094 PubMed40335722

2025

Identification of putative baroreceptors in human aortic arch by histological and omics analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Yankey Yundung,Jaroslav Pelisek,Umberto Maccio,Zsuzsanna Varga,Houria Ech-Cherif,Enni Markkanen,Fabian Rössler,Ahmed Ouda,Omer Dzemali,Alexander Zimmermann,Benedikt Reutersberg

European journal of human genetics : EJHG 33:24-29 PubMed39169228

2024

Novel fetal phenotype of TAF8 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Golan Nadav,Marwan Odeh,Aviv Mesika,Yael Abarbanel Har-Tal,Moshe Goldfeld,Tania Zalatkin,Alejandro Livoff,Raghad Jeris Khoury,Inshirah Sgayer,Liat Ben-Sira,Limor Kalfon,Tzipora C Falik-Zaccai

Genes & development 38:738-754 PubMed39168636

2024

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jiun C Chen,Govinal Badiger Bhaskara,Yue Lu,Kevin Lin,Sharon Y R Dent

Molecular cell 78:785-793.e8 PubMed32229306

2020

TFIID Enables RNA Polymerase II Promoter-Proximal Pausing.

Applications

Unspecified application

Species

Unspecified reactive species

Charli B Fant,Cecilia B Levandowski,Kapil Gupta,Zachary L Maas,John Moir,Jonathan D Rubin,Andrew Sawyer,Meagan N Esbin,Jenna K Rimel,Olivia Luyties,Michael T Marr,Imre Berger,Robin D Dowell,Dylan J Taatjes
View all publications
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Product promise

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