Rabbit Recombinant Monoclonal TAF9 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Western blot - Anti-TAF9 antibody [EPR12075(B)] (AB169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--western-blot-img116531.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAF9 antibody [EPR12075(B)] (AB169784)")
![Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--immunocytochemistry-immunofluorescence-img12584.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)")
![Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--immunocytochemistry-immunofluorescence-img109156.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)")
Publications
Filter by
- Communications biology 3:7902020Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Yao et. al.PubMed 33340029
- Molecular medicine reports 17:3226-32312017Cancer‑testis antigen HCA587/MAGEC2 interacts with the general transcription coactivator TAF9 in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPumei Zeng,Ying Wang,Yutian Zheng,Xiao Song,Yanhui YinPubMed 29257297
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Expected | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Expected | Not recommended | Predicted |
| Cow | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Orangutan | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/100 - 1/250 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF9 is also a component of the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:15899866). TAF9 and its paralog TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes (PubMed:15899866). Essential for cell viability (PubMed:15899866). May have a role in gene regulation associated with apoptosis (PubMed:15899866).
Alternative names
TAF2G, TAFII31, TAF9, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G, STAF31/32, Transcription initiation factor TFIID 31 kDa subunit, Transcription initiation factor TFIID 32 kDa subunit, TAFII-31, TAFII-32, TAFII32
Recommended products
Rabbit Recombinant Monoclonal TAF9 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12075(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAF9 also known as TATA-box binding protein-associated factor 9 is a component of the transcription factor IID (TFIID) complex that plays an essential role in the initiation of gene transcription by RNA polymerase II. This protein has a molecular mass of around 28 kDa and is primarily located in the nucleus of the cell. TAF9 is expressed in various tissues with notable expression levels in liver and kidney.
- Biological function summary
TAF9 associates with other proteins to form the TFIID complex an important component in the transcriptional machinery that binds to the TATA box region of the DNA. The interaction of TAF9 with TFIID facilitates the assembly of transcription initiation complexes at gene promoters impacting the transcription of numerous genes. This involvement in transcription regulation highlights TAF9's role in controlling cellular processes that depend on precise gene expression.
- Pathways
TAF9 is actively involved in the RNA polymerase II transcription initiation pathway and the signaling pathway related to gene expression. Within these pathways TAF9 interacts with other TAF proteins such as TAF1 and TAF4 ensuring efficient transcription regulation. Its role connects with broader transcriptional networks that manage cellular function and response to environmental cues.
- Associated diseases and disorders
Aberrant function or regulation of TAF9 is linked to liver cirrhosis and transcriptional dysregulation disorders. Changes in TAF9 expression can affect its interactions with components like TAF1 leading to disrupted transcriptional activities and potential disease progression. Understanding TAF9's connections to these diseases may offer insights for therapeutic strategies targeting transcription-related abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-TAF9 antibody [EPR12075(B)] (ab169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--western-blot-img116531.jpg "Western blot - Anti-TAF9 antibody [EPR12075(B)] (ab169784)")
Western blot - Anti-TAF9 antibody [EPR12075(B)] (ab169784)
All lanes: Western blot - Anti-TAF9 antibody [EPR12075(B)] (ab169784) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--immunocytochemistry-immunofluorescence-img12584.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784)")
Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TAF9 with purified ab169784 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
![Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784), expandable thumbnail](https://content.abcam.com/products/images/taf9-antibody-epr12075b-ab169784--immunocytochemistry-immunofluorescence-img109156.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784)")
Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (ab169784)
Immunofluorescence analysis of MCF7 cells, labeling TAF9 using ab169784 at a 1/100 dilution.
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