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AB169784

Anti-TAF9 antibody [EPR12075(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal TAF9 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

TAF2G, TAFII31, TAF9, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G, STAF31/32, Transcription initiation factor TFIID 31 kDa subunit, Transcription initiation factor TFIID 32 kDa subunit, TAFII-31, TAFII-32, TAFII32

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TAF9 with purified ab169784 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAF9 antibody [EPR12075(B)] (AB169784)

Immunofluorescence analysis of MCF7 cells, labeling TAF9 using ab169784 at a 1/100 dilution.

Western blot - Anti-TAF9 antibody [EPR12075(B)] (AB169784)
  • WB

Unknown

Western blot - Anti-TAF9 antibody [EPR12075(B)] (AB169784)

All lanes:

Western blot - Anti-TAF9 antibody [EPR12075(B)] (ab169784) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

false

  • Carrier free

    Anti-TAF9 antibody [EPR12075(B)] - BSA and Azide free

  • HRP

    HRP Anti-TAF9 antibody [EPR12075(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12075(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF9 also known as TATA-box binding protein-associated factor 9 is a component of the transcription factor IID (TFIID) complex that plays an essential role in the initiation of gene transcription by RNA polymerase II. This protein has a molecular mass of around 28 kDa and is primarily located in the nucleus of the cell. TAF9 is expressed in various tissues with notable expression levels in liver and kidney.
Biological function summary

TAF9 associates with other proteins to form the TFIID complex an important component in the transcriptional machinery that binds to the TATA box region of the DNA. The interaction of TAF9 with TFIID facilitates the assembly of transcription initiation complexes at gene promoters impacting the transcription of numerous genes. This involvement in transcription regulation highlights TAF9's role in controlling cellular processes that depend on precise gene expression.

Pathways

TAF9 is actively involved in the RNA polymerase II transcription initiation pathway and the signaling pathway related to gene expression. Within these pathways TAF9 interacts with other TAF proteins such as TAF1 and TAF4 ensuring efficient transcription regulation. Its role connects with broader transcriptional networks that manage cellular function and response to environmental cues.

Aberrant function or regulation of TAF9 is linked to liver cirrhosis and transcriptional dysregulation disorders. Changes in TAF9 expression can affect its interactions with components like TAF1 leading to disrupted transcriptional activities and potential disease progression. Understanding TAF9's connections to these diseases may offer insights for therapeutic strategies targeting transcription-related abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF9 is also a component of the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed : 15899866). TAF9 and its paralog TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes (PubMed : 15899866). Essential for cell viability (PubMed : 15899866). May have a role in gene regulation associated with apoptosis (PubMed : 15899866).
See full target information TAF9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 3:790 PubMed33340029

2020

Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Yao,Jia Yang,Qian Qin,Chao Tang,Zhidan Li,Li Chen,Kailong Li,Chunyan Ren,Lu Chen,Shuquan Rao

Molecular medicine reports 17:3226-3231 PubMed29257297

2017

Cancer‑testis antigen HCA587/MAGEC2 interacts with the general transcription coactivator TAF9 in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pumei Zeng,Ying Wang,Yutian Zheng,Xiao Song,Yanhui Yin
View all publications
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Product promise

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