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Rabbit Monoclonal Tafazzin / TAZ antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (AB307148), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (AB307148), expandable thumbnail
  • Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] (AB307148), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (AB307148), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (AB307148), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Not recommended
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Target data

Function

Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function (PubMed:12930833, PubMed:19164547, PubMed:19700766, PubMed:26908608, PubMed:33096711). Its role in cellular physiology is to improve mitochondrial performance (PubMed:32234310). CL is critical for the coassembly of lipids and proteins in mitochondrial membranes, for instance, remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms (By similarity). Catalyzes the transacylation between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor. CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization (By similarity). Required for the initiation of mitophagy (PubMed:33096711). Required to ensure progression of spermatocytes through meiosis (By similarity). Exon 7 of human tafazzin is essential for catalysis (PubMed:19700766). Isoform 1. Catalyzes the transacylation between lysophosphatidate (such as 1-acyl-sn-glycero-3-phosphate) and phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)) (PubMed:19700766). Contributes to cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL) remodeling (PubMed:12930833, PubMed:19700766). Isoform 3. Catalyzes the transacylation between lysophospholipids and phospholipids, and plays a fundamental role in cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL) metabolism and remodeling. Isoform 5. Catalytically inactive. Isoform 7. Catalytically inactive.

Alternative names

EFE2, G4.5, TAZ, TAFAZZIN, Tafazzin, Taz, Protein G4.5

Recommended products

Rabbit Monoclonal Tafazzin / TAZ antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26956-58
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tafazzin also known as TAZ is a mitochondrial phospholipid-lysophospholipid transacylase enzyme. It plays a role in the remodeling of cardiolipin a phospholipid found predominantly in the mitochondrial inner membrane. Tafazzin has a molecular mass of approximately 33 kDa. It is expressed in various tissues with higher expression levels found in tissues with high energy demands such as the heart and skeletal muscles.

Biological function summary

Tafazzin is essential for maintaining mitochondrial function and energy production. It is a critical component of the system responsible for cardiolipin metabolism. The enzyme does not work alone; it interacts with other mitochondrial proteins to facilitate the proper composition of cardiolipin species necessary for mitochondrial structural integrity and functionality.

Pathways

Tafazzin serves an important role in various lipid metabolism pathways. It participates in the phospholipid remodeling pathway where it interacts closely with acyltransferases to modify cardiolipin. This interaction is important for mitochondrial processes such as oxidative phosphorylation and energy conversion affecting proteins like cytochrome c oxidase and ATP synthase complex.

Associated diseases and disorders

Tafazzin mutations are linked to Barth syndrome a rare genetic condition that affects the heart and muscles. This disorder is marked by symptoms including cardiomyopathy muscle weakness and neutropenia. Tafazzin dysfunction interrupts normal cardiolipin remodeling impacting proteins such as cytochrome c which contributes to abnormal energy metabolism seen in the syndrome. Additionally altered Tafazzin activity has connections to mitochondrial dysfunctions observed in some cardiomyopathies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeti Tafazzin/TAZ whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Observed band size: 30 kDa

    Exposure time: 180s

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148) at 1/1000 dilution

    All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Observed band size: 30 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148), expandable thumbnail

    Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148)

    Tafazzin/TAZ was immunoprecipitated from 0.35 mg SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug with ab307148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307148 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug
    Lane 2: ABAB307148 IP in SW-13 whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307148 in SW-13 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148) at 1/1000 dilution

    Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 μg

    Lane 2: SW-13 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: Lanes 1, 4 and 5: 180 seconds, Lanes 2 and 3: 37 seconds

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148) at 1/1000 dilution

    Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) non-mitochondrial fraction 20 μg

    Lane 2: SW-13 mitochondria fraction 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (ab307148) at 1/1000 dilution

    Lane 1: Human colon cancer tissue lysate 20 μg

    Lane 2: Mouse heart tissue lysate 20 μg

    Lane 3: Mouse skeletal muscle tissue lysate 20 μg

    Lane 4: Rat heart tissue lysate 20 μg

    Lane 5: Rat skeletal muscle tissue lysate 20 μg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

    Lane 7: RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

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Product protocols

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