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Rabbit Monoclonal Tafazzin / TAZ antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

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Images

Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (AB307149), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (AB307149), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (AB307149), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (AB307149), expandable thumbnail
  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (AB307149), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Not recommended
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Target data

Function

Acyltransferase which is required to maintain the composition of the phospholipid cardiolipin, a key component of the mitochondrial inner membrane (PubMed:12930833, PubMed:19700766, PubMed:19164547, PubMed:26908608, PubMed:33096711). Required for the initiation of mitophagy (PubMed:33096711). Required to ensure progression of spermatocytes through meiosis (By similarity).Isoform 5Catalytically inactive.Isoform 7Catalytically inactive.

Alternative names

Tafazzin, Protein G4.5, TAZ, G4.5, EFE2, TAFAZZIN

Recommended products

Rabbit Monoclonal Tafazzin / TAZ antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

Alternative names

Tafazzin, Protein G4.5, TAZ, G4.5, EFE2, TAFAZZIN

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR26956-58

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tafazzin also known as TAZ is a mitochondrial phospholipid-lysophospholipid transacylase enzyme. It plays a role in the remodeling of cardiolipin a phospholipid found predominantly in the mitochondrial inner membrane. Tafazzin has a molecular mass of approximately 33 kDa. It is expressed in various tissues with higher expression levels found in tissues with high energy demands such as the heart and skeletal muscles.

Biological function summary

Tafazzin is essential for maintaining mitochondrial function and energy production. It is a critical component of the system responsible for cardiolipin metabolism. The enzyme does not work alone; it interacts with other mitochondrial proteins to facilitate the proper composition of cardiolipin species necessary for mitochondrial structural integrity and functionality.

Pathways

Tafazzin serves an important role in various lipid metabolism pathways. It participates in the phospholipid remodeling pathway where it interacts closely with acyltransferases to modify cardiolipin. This interaction is important for mitochondrial processes such as oxidative phosphorylation and energy conversion affecting proteins like cytochrome c oxidase and ATP synthase complex.

Associated diseases and disorders

Tafazzin mutations are linked to Barth syndrome a rare genetic condition that affects the heart and muscles. This disorder is marked by symptoms including cardiomyopathy muscle weakness and neutropenia. Tafazzin dysfunction interrupts normal cardiolipin remodeling impacting proteins such as cytochrome c which contributes to abnormal energy metabolism seen in the syndrome. Additionally altered Tafazzin activity has connections to mitochondrial dysfunctions observed in some cardiomyopathies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149), expandable thumbnail

    Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149)

    This data was developed using Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148, the same antibody clone in a different buffer formulation.
    Tafazzin/TAZ was immunoprecipitated from 0.35 mg SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug with Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug
    Lane 2: ABAB307148 IP in SW-13 whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 in SW-13 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-Tafazzin/TAZ antibody [EPR26956-58] (Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148) at 1/1000 dilution

    Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 μg

    Lane 2: SW-13 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

    Tafazzin/TAZ was immunoprecipitated from 0.35 mg SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug with Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) whole cell lysate 10 ug
    Lane 2: ABAB307148 IP in SW-13 whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148 in SW-13 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 180 seconds

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149)

    This data was developed using 307148, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration:
    Lanes 1, 4 and 5: 180 seconds, Lanes 2 and 3: 37 seconds
    Exposure time:

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148) at 1/1000 dilution

    Lane 1: SW-13 (human adrenal gland; cortex epithelial cell) non-mitochondrial fraction 20 μg

    Lane 2: SW-13 mitochondria fraction 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: Lanes 1, 4 and 5: 180 seconds, Lanes 2 and 3: 37 seconds

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149)

    This data was developed using 307148, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration:
    180 seconds
    Exposure time:

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148) at 1/1000 dilution

    Lane 1: Human colon cancer tissue lysate 20 μg

    Lane 2: Mouse heart tissue lysate 20 μg

    Lane 3: Mouse skeletal muscle tissue lysate 20 μg

    Lane 4: Rat heart tissue lysate 20 μg

    Lane 5: Rat skeletal muscle tissue lysate 20 μg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

    Lane 7: RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 30 kDa

    Exposure time: 180s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 180 seconds

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149)

    This data was developed using 307148, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: This blot was developed using a high sensitivity ECL substrate.

    180 seconds
    Exposure time:

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

    Lane 2: HeLa transfected with siRNA specifically targeti Tafazzin/TAZ whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Observed band size: 30 kDa

    Exposure time: 180s

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.

    Exposure time: 180 seconds

  • Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149), expandable thumbnail

    Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] - BSA and Azide free (ab307149)

    This data was developed using 307148, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: This blot was developed using a high sensitivity ECL substrate.

    180 seconds
    Exposure time:

    All lanes: Western blot - Anti-Tafazzin/TAZ antibody [EPR26956-58] (Anti-Tafazzin/TAZ antibody [EPR26956-58] ab307148) at 1/1000 dilution

    All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Observed band size: 30 kDa

    Exposure time: 180s

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.

    Exposure time: 180 seconds

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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