Mouse Multiclonal MCES antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human, Rat, Cow, Dog samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (AB199002), expandable thumbnail](https://content.abcam.com/products/images/tag-72-antibody-b723-cc49-ab199002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (AB199002)")
Publications
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- Theranostics 12:3150-31772022Glycoproteogenomics characterizes the CD44 splicing code associated with bladder cancer invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristiana Gaiteiro,Janine Soares,Marta Relvas-Santos,Andreia Peixoto,Dylan Ferreira,Paula Paulo,Andreia Brandão,Elisabete Fernandes,Rita Azevedo,Carlos Palmeira,Rui Freitas,Andreia Miranda,Hugo Osório,Jesús Prieto,Luís Lima,André M N Silva,Lúcio Lara Santos,José Alexandre FerreiraPubMed 35547758
- Journal of experimental & clinical cancer research : CR 40:1912021Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreia Peixoto,Dylan Ferreira,Rita Azevedo,Rui Freitas,Elisabete Fernandes,Marta Relvas-Santos,Cristiana Gaiteiro,Janine Soares,Sofia Cotton,Beatriz Teixeira,Paula Paulo,Luís Lima,Carlos Palmeira,Gabriela Martins,Maria José Oliveira,André M N Silva,Lúcio Lara Santos,José Alexandre FerreiraPubMed 34108014
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Rat | Expected | Expected | Expected |
| Caenorhabditis elegans | Predicted | Predicted | Predicted |
| Cow | Expected | Expected | Expected |
| Dog | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
Species Cow | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
Species Dog | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Cow | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Dog | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Species Rat | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Species Cow | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Species Dog | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Target data
Alternative names
CA 72.4, Tumor associated glycoprotein 72
Recommended products
Mouse Multiclonal MCES antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human, Rat, Cow, Dog samples. Cited in 2 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Clone number
- B72.3 + CC49
- Purification technique
- Affinity purification Protein G
- Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Both antibodies (B72.3 + CC49) are mouse IgG1 kappa.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (ab199002), expandable thumbnail](https://content.abcam.com/products/images/tag-72-antibody-b723-cc49-ab199002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (ab199002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (ab199002)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling tag-72 with ab199002 at 2 ug/ml.
Downloads
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