AB199002

Anti-tag-72 antibody [B72.3 + CC49]

Name: Anti-tag-72 antibody [B72.3 + CC49] (ab199002) | Abcam
Brand: Abcam Limited
SKU: AB199002
Price: 510 USD
Availability: InStock

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(6 Publications)

Mouse Multiclonal MCES antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human, Rat, Cow, Dog samples. Cited in 6 publications.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (AB199002)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (AB199002)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling tag-72 with ab199002 at 2 ug/ml.

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

B72.3 + CC49

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human, Dog, Rat, Cow

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Specificity

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Caenorhabditis elegans": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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Product details

Both antibodies (B72.3 + CC49) are mouse IgG1 kappa.

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information tag-72

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9375-9398 PubMed41041068

2025

Trimmed -glycans define aggressive gastric cancer and predict clinical outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan Ferreira,Beatriz Marinho-Santos,Marta Relvas-Santos,Bernardo Orr,Andreia Brandão,Luís Pedro Afonso,Lúcio Lara Santos,José Alexandre Ferreira

iScience 28:111758 PubMed39906564

2025

Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Peixoto,Dylan Ferreira,Andreia Miranda,Marta Relvas-Santos,Rui Freitas,Tim S Veth,Andreia Brandão,Eduardo Ferreira,Paula Paulo,Marta Cardoso,Cristiana Gaiteiro,Sofia Cotton,Janine Soares,Luís Lima,Filipe Teixeira,Rita Ferreira,Carlos Palmeira,Albert J R Heck,Maria José Oliveira,André M N Silva,Lúcio Lara Santos,José Alexandre Ferreira

International journal of molecular sciences 25: PubMed38542435

2024

Aberrantly Glycosylated GLUT1 as a Poor Prognosis Marker in Aggressive Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Ferreira,Dylan Ferreira,Marta Relvas-Santos,Rui Freitas,Janine Soares,Rita Azevedo,Luís Pedro Afonso,Luís Lima,Beatriz Santos,Martina Gonçalves,André M N Silva,Lúcio Lara Santos,Andreia Peixoto,José Alexandre Ferreira

Journal of gastrointestinal oncology 13:1959-1966 PubMed36092319

2022

The role of tumor-infiltrating B cells in the tumor microenvironment of hepatocellular carcinoma and its prognostic value: a bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jixue Zou,Chubin Luo,Haoyang Xin,Tongchun Xue,Xiaoying Xie,Rongxin Chen,Lan Zhang

Theranostics 12:3150-3177 PubMed35547758

2022

Glycoproteogenomics characterizes the CD44 splicing code associated with bladder cancer invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Gaiteiro,Janine Soares,Marta Relvas-Santos,Andreia Peixoto,Dylan Ferreira,Paula Paulo,Andreia Brandão,Elisabete Fernandes,Rita Azevedo,Carlos Palmeira,Rui Freitas,Andreia Miranda,Hugo Osório,Jesús Prieto,Luís Lima,André M N Silva,Lúcio Lara Santos,José Alexandre Ferreira

Journal of experimental & clinical cancer research : CR 40:191 PubMed34108014

2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Peixoto,Dylan Ferreira,Rita Azevedo,Rui Freitas,Elisabete Fernandes,Marta Relvas-Santos,Cristiana Gaiteiro,Janine Soares,Sofia Cotton,Beatriz Teixeira,Paula Paulo,Luís Lima,Carlos Palmeira,Gabriela Martins,Maria José Oliveira,André M N Silva,Lúcio Lara Santos,José Alexandre Ferreira

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-tag-72 antibody [B72.3 + CC49]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-tag-72 antibody [B72.3 + CC49] (AB199002)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise