Tag antibodies
Streamline your protein detection and purification workflows with our high-performance tag antibodies, optimized for FLAG, His-tag, GST, and GFP. These tools are part of our portfolio of over 60k primary antibodies, which are validated with over 600k citations. They will save you time and hassle by delivering unrivaled specificity and high recovery rates, ensuring your tagged proteins are captured with confidence.
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Tag antibodies are crucial tools used for the detection, quantification, and purification of proteins that have been tagged genetically or chemically. These tags could be small peptides, enzymes, or fluorescent proteins, which are recognized by tag-specific antibodies. Using these antibodies allows researchers and scientists to track the expression, assess the function, and analyze the location of tagged proteins within various biological systems.
Types of tag antibodies
Tag type
Uses
Key benefit
Examples
Small peptide
- Detection – Recognized by specific antibodies for western blot, immunofluorescence, ELISA.
- Purification – His-tag binds to nickel/cobalt resins for affinity purification.
- Localization – Track protein distribution in cells when protein-specific antibodies aren’t available.
Universal recognition across species and experiments, simplifying protein analysis.
Enzyme
- Detection – Recognized by specific antibodies for western blot, immunoprecipitation, ELISA.
- A ffinity purification – GST binds glutathione-coated resins, enabling efficient isolation of tagged proteins.
- Protein solubility & stability – Fusion with enzymes can improve folding and expression.
- Interaction studies – GST-tagged proteins are widely used in pull-down assays to identify binding partners.
Dual functionality—provides both a purification handle and a stabilizing fusion partner, making it ideal for protein expression and interaction studies.
Fluorescent
- Live-cell imaging – Visualize protein localization and dynamics in real time.
- Flow cytometry – Quantify tagged proteins in single cells.
- Co-localization studies – Track multiple proteins simultaneously using different fluorophores.
Direct visualization, ideal for dynamic studies.