AB133498

Anti-TAG1/TAX1 antibody [EPR5106]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal TAG1/TAX1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

AXT, TAG1, TAX1, CNTN2, Contactin-2, Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1

3 Images

Flow Cytometry (Intracellular) - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

Intracellular flow cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

All lanes:

Western blot - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 113 kDa

Observed band size: 135 kDa

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OI-RD Scanning - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

OI-RD Scanning

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OI-RD Scanning - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-TAG1/TAX1 antibody [EPR5106] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5106

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAG1 also known as TAX1 or contactin-2 is a protein encoded by the CNTN2 gene in humans. It is a member of the immunoglobulin superfamily and has a mass of approximately 140 kDa. TAG1 is expressed in the nervous system particularly in the peripheral and central nervous systems during development. It is predominantly found on neurons influencing axonal guidance and neuronal connectivity.

Biological function summary

TAG1 plays a fundamental role in the nervous system by mediating cell adhesion and signaling. It forms part of a complex often interacting with other cell adhesion molecules to facilitate cellular communication and neurite outgrowth. By influencing neuronal positioning and patterning TAG1 contributes to the proper formation of neural circuits.

Pathways

There is a strong involvement of TAG1 in axonal growth and guidance pathways as well as in the cell adhesion signaling pathway. It directly interacts with proteins such as Caspr and neurofascin which are critical for myelination and the maintenance of the node of Ranvier. These interactions help guide neuron development and function.

Altered expression of TAG1 has been linked to several neural disorders including autism spectrum disorders and schizophrenia. In the context of these diseases TAG1 interacts with other proteins such as contactin-associated protein 1 (Caspr1) which plays an important role in maintaining neural network stability and function. Understanding these interactions provides insights into the pathogenesis of these neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In conjunction with another transmembrane protein, CNTNAP2, contributes to the organization of axonal domains at nodes of Ranvier by maintaining voltage-gated potassium channels at the juxtaparanodal region. May be involved in cell adhesion.

See full target information CNTN2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3107 PubMed35661110

2022

Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoko Kuwayama,Keiichiro Suzuki,Jun Nakamura,Emi Aizawa,Yoshichika Yoshioka,Masahito Ikawa,Shin Nabatame,Ken-Ichi Inoue,Yoshiari Shimmyo,Keiichi Ozono,Taroh Kinoshita,Yoshiko Murakami

Molecular & cellular proteomics : MCP 17:1670-1684 PubMed29848781

2018

Discovery of Novel Cell Surface Markers for Purification of Embryonic Dopamine Progenitors for Transplantation in Parkinson's Disease Animal Models.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Fathi,Mehdi Mirzaei,Banafsheh Dolatyar,Mehdi Sharifitabar,Mahnaz Bayat,Ebrahim Shahbazi,Jaesuk Lee,Mohammad Javan,Su-Chun Zhang,Vivek Gupta,Bonghee Lee,Paul A Haynes,Hossein Baharvand,Ghasem Hosseini Salekdeh

Oncology letters 14:2137-2140 PubMed28789439

2017

Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Bo Zhen,Xiao-Feng Chen,Tao Yan,Shi-Guang Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAG1/TAX1 antibody [EPR5106]

Flow Cytometry (Intracellular) - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

Western blot - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

OI-RD Scanning - Anti-TAG1/TAX1 antibody [EPR5106] (AB133498)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise