AB250938

Anti-TAGAP antibody [EPR15593] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TAGAP antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

TAGAP1, FKSG15, TAGAP, T-cell activation Rho GTPase-activating protein, T-cell activation GTPase-activating protein

3 Images

Supplier Data

Western blot - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

This data was developed using ab187664, the same antibody clone in a different buffer formulation.

Based on sequence analysis, ab187664 recognizes three isoforms with the predicted MWs of 81kDa, 61kDa and 57kDa, respectively.

All lanes:

Western blot - Anti-TAGAP antibody [EPR15593] (<a href='/en-us/products/primary-antibodies/tagap-antibody-epr15593-ab187664'>ab187664</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 81 kDa

Observed band size: 61 kDa,81 kDa

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Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

This data was developed using ab187664, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TAGAP with ab187664 at 1/160 dilution (pink) compared to a Rabbit monoclonal IgG Isotype control (green). Goat anti rabbit IgG (FITC) was used as the secondary antibody at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

This data was developed using ab187664, the same antibody clone in a different buffer formulation.

Western blot analysis of TAGAP in A549 cell lysate immunoprecipitated with ab187664 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TAGAP antibody [EPR15593] (<a href='/en-us/products/primary-antibodies/tagap-antibody-epr15593-ab187664'>ab187664</a>)

Predicted band size: 81 kDa

false

Unconjugated

Anti-TAGAP antibody [EPR15593]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15593

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250938 is the carrier-free version of ab187664.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGAP also known as T-cell activation RhoGTPase activating protein acts as a Rho GTPase-activating protein involved in the regulation of the actin cytoskeleton. It weighs approximately 70 kDa. This protein is particularly expressed in immune cells including T-cells B-cells and dendritic cells. TAGAP plays an important role in the modulation of signaling pathways essential for immune cell activation and function.

Biological function summary

TAGAP contributes to the regulation of immune responses. It influences the rearrangement of the actin cytoskeleton affecting cell movement and activation. Though not a part of a large protein complex TAGAP's ability to manage cytoskeletal dynamics is critical in immune cells such as T-cells enabling proper response to antigens.

Pathways

TAGAP is significant in the T-cell receptor signaling and actin cytoskeleton regulation pathways. These pathways facilitate immune cell activation and migration key processes in immune response. TAGAP mediates interactions with proteins like Rac1 involved in cytoskeletal organization and Vav1 a guanine nucleotide exchange factor that activates pathways necessary for the immune response.

TAGAP's function links to autoimmune diseases like multiple sclerosis and rheumatoid arthritis. Altered TAGAP expression or function can influence disease susceptibility making it a target for research. Through autoimmune-related pathways TAGAP interacts with proteins such as IL-2 and STAT4 which are implicated in these disorders highlighting its potential role in influencing immune system balance and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as a GTPase-activating protein and may play important roles during T-cell activation.

See full target information TAGAP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAGAP antibody [EPR15593] - BSA and Azide free

Western blot - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

Immunoprecipitation - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (AB250938)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise