AB10135

Anti-TAGLN/Transgelin antibody

Name: Anti-TAGLN/Transgelin antibody (ab10135) | Abcam
Brand: Abcam Limited
SKU: AB10135
Price: 505 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(153 Publications)

Anti-TAGLN/Transgelin antibody (ab10135) is a goat polyclonal antibody detecting TAGLN/Transgelin in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rabbit, Rat.

- Over 130 publications
- Trusted since 2004

View Alternative Names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

1 Images

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Western blot - Anti-TAGLN/Transgelin antibody (AB10135)

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab10135) at 0.3 µg/mL

All lanes:

Human Testes lysate at 35 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse, Rabbit

Applications

WB, IHC-FoFr, IHC-Fr, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human TAGLN aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q01995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "Primary incubation for 1 hour at room temperature.", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TAGLN/Transgelin antibody (ab10135) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rabbit, Rat samples.

Tagged protein detection
TAGLN/Transgelin antibodies are used to visualize proteins labelled with this tag in a variety of applications.

What is the molecular weight of TAGLN/Transgelin?
Anti-TAGLN/Transgelin (ab10135) specifically detects a band for TAGLN/Transgelin (UniProt: Q01995) at a molecular weight of 23kDa.

Trusted by the scientific community
Anti-TAGLN/Transgelin (ab10135) was first used in a scientific publication in 2004 and has been cited over 130 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

See full target information TAGLN

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Publications (153)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869394

2025

BRD4 Mediates Transforming Growth Factor-β-Induced Smooth Muscle Cell Differentiation from Mesenchymal Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ayobami Olajuyin,Venkatakirankumar Mandlem,Christudas Sunil,Yunzhuan Hou,Oluwaseun Adeyanju,Sana Petkar,Qinying Li,Torry A Tucker,Steven Idell,Shi-You Chen,Xia Guo,Guoqing Qian

Toxins 17: PubMed40864097

2025

Kynurenine Promotes Phosphate-Induced Endothelial Calcification via Endothelial-to-Mesenchymal Transition, Osteoblastic Differentiation and AhR Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Molinaro,Mario Cozzolino,Paola Ciceri

JCI insight 10: PubMed40857411

2025

Single-cell spatial transcriptomics unravels the cellular landscape of abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhen Zhao,Chun-Seok Cho,Hongyu Liu,Yongha Hwang,Yichen Si,Myungjin Kim,Yongjie Deng,Yang Zhao,Chao Xue,Yanhong Guo,Lin Chang,Dogukan Mizrak,Bo Yang,Hyun Min Kang,Jifeng Zhang,Jun Hee Lee,Y Eugene Chen

Cells 14: PubMed40136697

2025

The Recovery of Epidermal Proliferation Pattern in Human Skin Xenograft.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Cherkashina,Alexandra Tsitrina,Danila Abolin,Elena Morgun,Anastasiya Kosykh,Marat Sabirov,Ekaterina Vorotelyak,Ekaterina Kalabusheva

Journal of pharmaceutical analysis 15:101120 PubMed39989903

2025

Hemodynamic disturbance and mTORC1 activation: Unveiling the biomechanical pathogenesis of thoracic aortic aneurysms in Marfan syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yuan Liu,Meili Wang,Junjun Liu,An-Qiang Sun,Chang-Shun He,Xin Cong,Wei Kong,Wei Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409250 PubMed39716891

2024

TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Hong Wu,Yu-Xiao Liu,Jia-Bin Zong,Min Qiu,Yi-Fan Zhou,Ya-Nan Li,Tuersun Aili,Xin-Ran Zhao,Bo Hu

Nature communications 15:7996 PubMed39266508

2024

Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Yang,Lihang Zhu,Shiya Wang,Jixing Gong,Jonathan Nimal Selvaraj,Lincai Ye,Hanxiao Chen,Yaoyao Zhang,Gongxin Wang,Wanjun Song,Zilong Li,Lin Cai,Hao Zhang,Donghui Zhang

Cell proliferation 58:e13742 PubMed39219022

2024

CircSMAD3 represses VSMC phenotype switching and neointima formation via promoting hnRNPA1 ubiquitination degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Mei,Xiaozhu Ma,Li Zhou,Qidamugai Wuyun,Ziyang Cai,Jiangtao Yan,Hu Ding

Journal of molecular and cellular cardiology 195:1-13 PubMed39038734

2024

Transient formation of collaterals contributes to the restoration of the arterial tree during cardiac regeneration in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Sturny,Lucie Boulgakoff,Robert G Kelly,Lucile Miquerol

iScience 27:109849 PubMed38784008

2024

TUDCA alleviates atherosclerosis by inhibiting AIM2 inflammasome and enhancing cholesterol efflux capacity in macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Wang,Yuesheng Zhang,Luping Du,Zhengchen Jiang,Yan Guo,Kai Wang,Yijiang Zhou,Xiang Yin,Xiaogang Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TAGLN/Transgelin antibody

Western blot - Anti-TAGLN/Transgelin antibody (AB10135)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (153)

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