AB137453

Anti-TAGLN/Transgelin antibody

Name: Anti-TAGLN/Transgelin antibody (ab137453) | Abcam
Brand: Abcam Limited
SKU: AB137453
Rating: 5 (3 reviews)

(3 Reviews)

(3 Publications)

Rabbit Polyclonal TAGLN/Transgelin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TAGLN aa 1 to C-terminus.

View Alternative Names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB137453)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB137453)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Transgelin protein at cytoplasm and nucleus using ab137453 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab137453) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab137453) at 1/5000 dilution

Lane 1:

TAGLN shRNA transfected HeLa whole cell extracts at 15 µg

Lane 2:

Non-transfected HeLa whole cell extracts at 15 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab137453) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab137453) at 1/1000 dilution

All lanes:

C8D30 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab137453) at 1/5000 dilution

All lanes:

Rat-2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TAGLN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

See full target information TAGLN

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Hepatology (Baltimore, Md.) 78:1092-1105 PubMed37055018

2023

Semaphorin 3C exacerbates liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca De Angelis Rigotti,Lena Wiedmann,Max Ole Hubert,Margherita Vacca,Sana S Hasan,Iris Moll,Silvia Carvajal,Wladimiro Jiménez,Maja Starostecka,Adrian T Billeter,Beat Müller-Stich,Gretchen Wolff,Bilgen Ekim-Üstünel,Stephan Herzig,Cristina Fandos-Ramo,Ralph Krätzner,Maria Reich,Verena Keitel-Anselmino,Mathias Heikenwälder,Carolin Mogler,Andreas Fischer,Juan Rodriguez-Vita

Scientific reports 9:18224 PubMed31796853

2019

Loss of the serine protease HTRA1 impairs smooth muscle cells maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Ralph Klose,Alexander Prinz,Fabian Tetzlaff,Eva-Maria Weis,Iris Moll,Juan Rodriguez-Vita,Chio Oka,Thomas Korff,Andreas Fischer

International journal of medical sciences 16:1149-1156 PubMed31523178

2019

The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zheng,Xiaotong Hu,Wang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAGLN/Transgelin antibody

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB137453)

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Western blot - Anti-TAGLN/Transgelin antibody (AB137453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise