Anti-TAGLN/Transgelin antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB14106)

ab14106 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed in 100% methanol for 5 minutes, permeabilized in 0.1% PBS-Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14106 at 1 μg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor^® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.

DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1 hour at room temperature.

• ICC/IF

AbReview42536****

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB14106)

ab14106 staining SM22 alpha in mouse muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000.

This image is courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Anti-TAGLN/Transgelin antibody (AB14106)

The band was completed abolished by peptide blocking with ab16067 (immunizing peptide) - not shown.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa nuclear extract at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

• WB

Ap21132****

Western blot - Anti-TAGLN/Transgelin antibody (AB14106)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab14106 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL

Lane 1:

Human colon tissue lysate - total protein (<a href='/en-us/products/unavailable/human-colon-tissue-lysate-total-protein-ab30051'>ab30051</a>) at 10 µg

Lane 2:

Mouse colon tissue lysate at 10 µg

Lane 3:

Rat colon tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 23 kDa

Observed band size: 115 kDa,20 kDa,23 kDa,36 kDa

true

Exposure time: 5s

• WB

AbReview16283****

Western blot - Anti-TAGLN/Transgelin antibody (AB14106)

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1/1000 dilution

All lanes:

Lysates prepared from human primary smooth muscle cells at 30 µg

Secondary

All lanes:

HRP-conjugated bovine polyclonal to rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

true

Exposure time: 2min

This image is a courtesy of Anonymous Abreview

• WB

Unknown

Western blot - Anti-TAGLN/Transgelin antibody (AB14106)

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human TAGLN/Transgelin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-tagln-transgelin-protein-ab101469'>ab101469</a>) at 0.01 µg

Lane 1:

Western blot - Recombinant Human SPG21/Maspardin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-spg21-maspardin-protein-ab86438'>ab86438</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 23 kDa,35 kDa

true

Exposure time: 30s