Anti-TAGLN/Transgelin antibody is a rabbit polyclonal antibody that is used to detect TAGLN/Transgelin in ICC/IF, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 430 publications
- Trusted since 2005
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- Disease models & mechanisms 17:2024eNOS plays essential roles in the developing heart and aorta linked to disruption of Notch signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorraine Eley,Rachel V Richardson,Ahlam Alqahtani,Bill Chaudhry,Deborah J HendersonPubMed 38111957
- Biochemistry and biophysics reports 36:1015672023Ischemia‒Reperfusion accelerates neointimal hyperplasia via IL-1β-mediated pyroptosis after balloon injury in the rat carotid artery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijun Wei,Runyu Liu,Ming Zhao,Yarong Ma,Yanzheng He,Xiaolei SunPubMed 37965065
- iScience 26:1083602023Treatment of calcific arterial disease via enhancement of autophagy using GSK343.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian L Lino Cardenas,Wanlin Jiang,Lova P Kajuluri,Kuldeep Singh,Katrina Ostrom,Rebecca Li,Francois Cherbonneau,Sophie Boerboom,Claire Birchenough,Kangsan Roh,Elizabeth L Chou,Zarbafian Shahrooz,Christopher Nicholson,Adam L Johnson,Sujin Lee,Fumito Ichinose,Donald B Bloch,Sagar Nigwekar,Patrick T Ellinor,Patricia Musolino,Mark E Lindsay,Zhixun Dou,Clint L Miller,Rajeev MalhotraPubMed 38033629
- Fluids and barriers of the CNS 20:762023SARS-CoV-2 infects epithelial cells of the blood-cerebrospinal fluid barrier rather than endothelial cells or pericytes of the blood-brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Stüdle,Hideaki Nishihara,Sven Wischnewski,Laila Kulsvehagen,Sylvain Perriot,Hiroshi Ishikawa,Horst Schroten,Stephan Frank,Nikolaus Deigendesch,Renaud Du Pasquier,Lucas Schirmer,Anne-Katrin Pröbstel,Britta EngelhardtPubMed 37875964
- Nutrients 15:2023Gut Microbiota-Derived Tryptophan Metabolite Indole-3-aldehyde Ameliorates Aortic Dissection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSui-Shane Huang,Rongle Liu,Shufu Chang,Xiao Li,Xinyu Weng,Junbo GePubMed 37836434
- International journal of nanomedicine 18:5011-50302023Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengshi Li,Zhihua Rong,Tianqi Chen,Peng Wang,Xiao Di,Leng Ni,Changwei LiuPubMed 37693888
- Cellular and molecular life sciences : CMLS 80:2642023SMYD2 regulates vascular smooth muscle cell phenotypic switching and intimal hyperplasia via interaction with myocardin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhou,Shaligram Sharma,Xiaonan Sun,Xiaoqing Guan,Yuning Hou,Zhe Yang,Hang Shi,Ming-Hui Zou,Ping Song,Jiliang Zhou,Shenming Wang,Zuojun Hu,Chunying LiPubMed 37615725
- Cell division 18:132023The roles of METTL3 on autophagy and proliferation of vascular smooth muscle cells are mediated by mTOR rather than by CDK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanshen Luo,Xingliang Wu,Bo Huo,Liyuan Liu,Ding-Sheng Jiang,Xin YiPubMed 37559091
- Theranostics 13:4059-40782023Soluble CD93 lectin-like domain sequesters HMGB1 to ameliorate inflammatory diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShang-En Huang,Cheng-Hsiang Kuo,Si-Yu Shiao,Chia-Rui Shen,Fang-Tzu Lee,Bi-Ing Chang,Jong-Hau Hsu,Hua-Lin Wu,Jwu-Lai Yeh,Chao-Han LaiPubMed 37554277
- Fluids and barriers of the CNS 20:512023Unique features of the arterial blood-brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBatia Bell,Shira Anzi,Esther Sasson,Ayal Ben-ZviPubMed 37370096
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/µL | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Alternative names
Sm22, Sm22a, Transgelin, Actin-associated protein p27, Smooth muscle protein 22-alpha, SM22-alpha
Recommended products
Anti-TAGLN/Transgelin antibody is a rabbit polyclonal antibody that is used to detect TAGLN/Transgelin in ICC/IF, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 430 publications
- Trusted since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunogen used to generate this TAGLN/Transgelin antibody (ab14106) shares 75% homology with family members TAGLN2 and TAGLN3. Cross-reactivity with these proteins has not been confirmed experimentally.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-TAGLN/Transgelin antibody (ab14106) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse, rat samples.
Quality and Validation
Anti-TAGLN/Transgelin antibody (ab14106) specifically detects TAGLN/Transgelin (UniProt ID: Q01995; Molecular weight: 22kDa) and is sold in 100 µg selling sizes.
Abcam's high quality validation processes ensure Anti-TAGLN/Transgelin antibody (ab14106) has high sensitivity and specificity.
Anti-TAGLN/Transgelin antibody (ab14106) has been cited over 435 times in peer reviewed journals and is trusted by the scientific community.
Anti-TAGLN/Transgelin antibody (ab14106) has 27 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)
ab14106 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol for 5 minutes, permeabilized in 0.1% PBS-Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14106 at 1 μg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1 hour at room temperature.
This image is a courtesy of Anonymous customer reviewWestern blot - Anti-TAGLN/Transgelin antibody (ab14106)
All lanes: Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1/1000 dilution
All lanes: Lysates prepared from human primary smooth muscle cells at 30 µg
SecondaryAll lanes: HRP-conjugated bovine polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 2min
This image is courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)
ab14106 staining SM22 alpha in mouse muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000.
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
The band was completed abolished by peptide blocking with ab16067 (immunizing peptide) - not shown.
All lanes: Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HeLa nuclear extract at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab14106 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL
Lane 1: Human colon tissue lysate - total protein (ab30051) at 10 µg
Lane 2: Mouse colon tissue lysate at 10 µg
Lane 3: Rat colon tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 115 kDa, 20 kDa, 23 kDa, 36 kDa
Exposure time: 5s
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
All lanes: Western blot - Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/mL
Lane 1: Western blot - Recombinant Human TAGLN/Transgelin protein (Recombinant Human TAGLN/Transgelin protein ab101469) at 0.01 µg
Lane 1: Western blot - Recombinant Human SPG21/Maspardin protein (Recombinant Human SPG21/Maspardin protein ab86438)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa, 35 kDa
Exposure time: 30s
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