AB155272

Anti-TAGLN/Transgelin antibody

Name: Anti-TAGLN/Transgelin antibody (ab155272) | Abcam
Brand: Abcam Limited
SKU: AB155272
Price: 440 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(18 Publications)

Rabbit Polyclonal TAGLN/Transgelin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TAGLN.

View Alternative Names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB155272)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB155272)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Transgelin protein at cytoplasm using ab155272 at a 1/100 dilution (Green). alpha Tubulin, a cytoskeleton marker was stained at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue stained for Transgelin protein at cytosol on mouse heart with ab155272 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue stained for Transgelin protein at cytoplasm with ab155272 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue stained for Transgelin protein at cytosol on mouse esophagus with ab155272 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Supplier Data

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

12% SDS PAGE

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody (ab155272) at 1/5000 dilution

Lane 1:

A-10 whole cell lysate at 35 µg

Lane 2:

A-10 whole cell lysate treated with MG132 at 35 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TAGLN. The exact immunogen used to generate this antibody is proprietary information.

Q01995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

See full target information TAGLN

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114837 PubMed39368089

2024

Volumetric analysis of the terminal ductal lobular unit architecture and cell phenotypes in the human breast.

Applications

Unspecified application

Species

Unspecified reactive species

Oona Paavolainen,Markus Peurla,Leena M Koskinen,Jonna Pohjankukka,Kamyab Saberi,Ella Tammelin,Suvi-Riitta Sulander,Masi Valkonen,Larissa Mourao,Pia Boström,Nina Brück,Pekka Ruusuvuori,Colinda L G J Scheele,Pauliina Hartiala,Emilia Peuhu

Cancers 16: PubMed39335130

2024

Cancer-Associated Fibroblast Proteins as Potential Targets against Colorectal Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ruchi Shah,Katherine A Johnson,Anna E L Lippert,Sean G Kraus,Philip B Emmerich,Cheri A Pasch,Wei Zhang,Kristina A Matkowskyj,Aaron M LeBeau,Dustin A Deming

Scientific reports 14:559 PubMed38177414

2024

WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zengshi Li,Junqiang Huang,Lijian Yang,Xi Li,Wei Li

International journal of molecular sciences 24: PubMed37373164

2023

Empagliflozin Attenuates Vascular Calcification in Mice with Chronic Kidney Disease by Regulating the NFR2/HO-1 Anti-Inflammatory Pathway through AMPK Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Wen Lu,Chung-Jen Lee,Yi-Jen Hsieh,Bang-Gee Hsu

Cell discovery 8:92 PubMed36104333

2022

Single-cell profiling reveals molecular basis of malignant phenotypes and tumor microenvironments in small bowel adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Yang,Xin Zhou,Ji Dong,Wendong Wang,Yongqu Lu,Yuan Gao,Yu Zhang,Yunuo Mao,Junpeng Gao,Wei Wang,Qingqing Li,Shuai Gao,Lu Wen,Wei Fu,Fuchou Tang

Matrix biology plus 12:100085 PubMed34693248

2021

Deletion of type VIII collagen reduces blood pressure, increases carotid artery functional distensibility and promotes elastin deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda L Mohabeer,Jeffrey T Kroetsch,Meghan McFadden,Negin Khosraviani,Thomas J Broekelmann,Guangpei Hou,Hangjun Zhang,Yu-Qing Zhou,Minyao Wang,Anthony O Gramolini,Robert P Mecham,Scott P Heximer,Steffen-Sebastian Bolz,Michelle P Bendeck

Journal of cardiovascular pharmacology 78:308-318 PubMed34091481

2021

Curcumin-mediated Photodynamic Therapy Inhibits the Phenotypic Transformation, Migration, and Foaming of Oxidized Low-density Lipoprotein-treated Vascular Smooth Muscle Cells by Promoting Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Gailan Wang,Ying Zhu,Kaiting Li,Bo Liao,Fang Wang,Lan Shao,Liyi Huang,Dingqun Bai

Life sciences 272:119232 PubMed33600866

2021

MSC-derived exosomal lncRNA SNHG7 suppresses endothelial-mesenchymal transition and tube formation in diabetic retinopathy via miR-34a-5p/XBP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Cao,Li-Dan Xue,Yue Di,Tao Li,Ya-Jing Tian,Yu Song

Journal of gastrointestinal oncology 11:1242-1252 PubMed33456997

2021

Crocin reverses 1-methyl-3-nitroso-1-nitroguanidine (MNNG)-induced malignant transformation in GES-1 cells through the Nrf2/Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhide Wu,Jianping Hui

Stem cells and development 29:981-993 PubMed32486904

2020

Human Umbilical Cord Mesenchymal Stem Cells Attenuate Abdominal Aortic Aneurysm Progression in Sprague-Dawley Rats: Implication of Vascular Smooth Muscle Cell Phenotypic Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wen,Mingjing Wang,Shiqiang Gong,Xintong Li,Jinze Meng,Jie Wen,Yifei Wang,Shuqing Zhang,Shijie Xin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAGLN/Transgelin antibody

Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody (AB155272)

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

Western blot - Anti-TAGLN/Transgelin antibody (AB155272)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise