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Rabbit Recombinant Monoclonal TAGLN/Transgelin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (AB249579), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (AB249579), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (AB249579), expandable thumbnail
  • Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (AB249579), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (AB249579), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Rat
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Target data

Function

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

Alternative names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

Recommended products

Rabbit Recombinant Monoclonal TAGLN/Transgelin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11995(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249579 is the carrier-free version of Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Associated diseases and disorders

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling TAGLN/Transgelin using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human smooth vessels tissue, labeling TAGLN/Transgelin using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling TAGLN/Transgelin using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902) at 1/1000 dilution

    Lane 1: Human bladder lysate at 10 µg

    Lane 2: Human stomach lysate at 10 µg

    Lane 3: Human uterus lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal mucle with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle.

    The section was incubated with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skeletal mucle with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on Rat skeletal muscle.

    The section was incubated with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human stomach labelling Human stomach with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle in human stomach.


    The section was incubated with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon labelling TAGLN/Transgelin with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle in Rat colon.


    The section was incubated with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST

    Negative control: skeletal muscle.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Lane 3: Rat colon tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Predicted band size: 23 kDa

    Observed band size: 22 kDa

    Exposure time: 6s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)

    This data was developed using Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human esophagus labelling TAGLN/Transgelin with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle in human esophagus.


    The section was incubated with Anti-TAGLN/Transgelin antibody [EPR11995(B)] ab170902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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