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Human Recombinant Monoclonal TAGLN/Transgelin antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (AB213273), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (AB213273), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (AB213273), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (AB213273), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (AB213273), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Human

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1.00000-5.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1.00000-5.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1.00000-5.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1.00000-2.50000 µg/mL

Notes

-

Species

Rat

Dilution info

1.00000-2.50000 µg/mL

Notes

-

Species

Human

Dilution info

1.00000-2.50000 µg/mL

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

Alternative names

SM22, WS3-10, TAGLN, SM22, WS3-10, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

Recommended products

Human Recombinant Monoclonal TAGLN/Transgelin antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21206

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.

Human monoclonal antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Associated diseases and disorders

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Human IgG H&L (IRDye® 800CW) preabsorbed secondary at 1/10000 dilution for 1 hour.

    All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273) at 1 µg/mL

    Lane 1: Human skeletal muscle tissue lysate at 10 µg

    Lane 2: Human colon tissue lysate at 10 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 10 µg

    Lane 4: Mouse Colon tissue lysate at 10 µg

    Lane 5: Mouse Bladder Tissue Lysate at 10 µg

    Secondary

    All lanes: Goat anti-Human IgG H&L (IRDye® 800CW) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    IHC image of SM22 alpha staining in a section of formalin-fixed paraffin-embedded normal mouse bladder performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213273, 5ug/ml, for 15 mins at room temperature.

    An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    IHC image of SM22 alpha staining in a section of formalin-fixed paraffin-embedded normal human bladder* performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213273, 5ug/ml, for 15 mins at room temperature.

    An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) secondary antibody at 1/1000 dilution. Negative control: no staining in rat skeletal muscle. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat lung. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    Immunohistochemical analysis of paraffin-embedded rat esophagus tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat esophagus. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273), expandable thumbnail

    Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273)

    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Exposure Time: Lane 1: 3 seconds, Lane 2-3: 20 seconds.

    Negative control: Skeletal muscle.

    The identity of the bands between 27 kDa and 150 kDa are unknown.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.

    All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273) at 1/500 dilution

    Lane 1: Rat bladder tissue lysate at 20 µg

    Lane 2: Rat colon tissue lysate at 20 µg

    Lane 3: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (Goat Anti-Human IgG Fc (HRP) preadsorbed ab98624) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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