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Rabbit Recombinant Monoclonal TAGLN/Transgelin antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000

Notes

-

Species

Rat

Dilution info

1/10000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

Alternative names

SM22, WS3-10, TAGLN, SM22, WS3-10, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

Recommended products

Rabbit Recombinant Monoclonal TAGLN/Transgelin antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21215

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Associated diseases and disorders

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566), expandable thumbnail

    Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with Anti-TAGLN/Transgelin antibody [EPR21206] ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 secondary at 1/10000 dilution for 1 hour.

    All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/mL

    Lane 1: Human Skeletal Muscle tissue lysate at 10 µg

    Lane 2: Human Colon tissue lysate at 10 µg

    Lane 3: Mouse Skeletal Muscle tissue lysate at 10 µg

    Lane 4: Mouse Colon tissue lysate at 10 µg

    Lane 5: Mouse Bladder tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: No staining on rat skeletal muscle.

    The section was incubated with ab227566 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: No staining on mouse skeletal muscle.

    The section was incubated with ab227566 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

    Immunohistochemical analysis of paraffin-embedded mouse colon labelling TAGLN/Transgelin with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Positive staining on smooth muscle in mouse colon

    The section was incubated with ab227566 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566)

    Immunohistochemical analysis of paraffin-embedded rat colon labelling TAGLN/Transgelin with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Positive staining on smooth muscle in rat colon

    The section was incubated with ab227566 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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