Anti-TAGLN/Transgelin antibody [EPR21215]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : No staining on mouse skeletal muscle.

The section was incubated with ab227566 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566)

Immunohistochemical analysis of paraffin-embedded mouse colon labelling TAGLN/Transgelin with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Positive staining on smooth muscle in mouse colon
The section was incubated with ab227566 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566)

Immunohistochemical analysis of paraffin-embedded rat colon labelling TAGLN/Transgelin with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Positive staining on smooth muscle in rat colon
The section was incubated with ab227566 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle with ab227566 at 1/10000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : No staining on rat skeletal muscle.

The section was incubated with ab227566 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Lab

Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (AB227566)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 secondary at 1/10000 dilution for 1 hour.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/mL

Lane 1:

Human Skeletal Muscle tissue lysate at 10 µg

Lane 2:

Human Colon tissue lysate at 10 µg

Lane 3:

Mouse Skeletal Muscle tissue lysate at 10 µg

Lane 4:

Mouse Colon tissue lysate at 10 µg

Lane 5:

Mouse Bladder tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa

false